Question

Can someone answer these questions please!? Need help!!!!

    1.   Compare   the   optimum   pH   results   obtained   for   amylase   and   pepsin   with   those   predicted   in   the   Pre-Laboratory Assignment.   Discuss   any   possible   discrepancies   between   the   predicted   and   experimental   results.
   2.   What   was   the   purpose   of   including   control   samples   containing   only   substrate   and   buffer   (test   tubes   1−4   for   each   enzyme)   in   this   study?   Did   any   of   these   mixtures   show   any   reaction?
   3.   Protein   digestion   involves   the   hydrolysis   of   peptide   linkages   and   is   thus   the   reverse   of   protein   synthesis,   in   which   amino   acids   combine.   Define   the   term   hydrolysis and complete the following equation for the hydrolysis of a dipeptide.
H3N CH CH3
C
CH2OH NH CH
O
CO2– + H2O H3N CH CH3
CH2OH CHCO 2 – + H3N CO2– + + +
Digestive Enzymes continued
8 Laboratory Experiments for General, Organic and Biological Chemistry
4. The protease enzymes pepsin, trypsin and chymotrypsin are released in inactive forms called zymogens that must be   cleaved   and   activated   by   hydrolysis   prior   to   their   role   in   protein   digestion.   How   does   this   cleavage   step   help   to   regulate   enzyme   activity?
5. Manufacturers of nutritional supplements claim that ingesting powdered enzymes will increase the ability of the body   to   digest   food.   Critique   (defend   or   criticize)   this   marketing   position

Answer 1

Answer 1:)

Pepsin works at the optimum pH of 1.5 to 1.6 while pancreatic and salivary amylase needs around neutral pH for the optimum work i.e. 6.7 - 7.0. The experimental values can be different due to the presence of excess NaOH or HCl during pH calibration.

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