Question

16. During electrophoresis, the gas that comes out at the positive end is: (3 pts) 17. During electrophoresis, the gas that comes out at the negative end is: (3 pts) 18. The inducer that directly causes expression of the GFP protein on the pGLO plasmid is: (3 pts) 19. A circular strand of DNA is about 5000 bps long. The enzyme Hind III cuts this plasmid at position 1500, 2700, 3600 and 4000. Predict how many fragments will appear on the gel after the digest and calculate the sizes of these fragments. (10 points) 20. Suppose you are given some human pancreatic cells and some E. coli and all the lab tools and resources you may get in a standard molecular biology lab. Using knowledge from the entire course, discuss how you may make the E. coli you have to produce human insulin from the given human pancreatic cells (10 points)

Answer 1

16. Oxygen
2H2O → O, + 4H+ + 4e- Anode

17. Hydrogen
-Cathode

18. Arabinose

GFP will only be expressed in presence of arabinose, and is regulated by araC (the promoter region).

19. Total five fragments will appear. Sizes will be 1500 bp, 1200 bp, 900 bp, 400 bp and 1000 bp.

1500 27 o3G coo (Hind m СОС