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2. Tonicity. (a) How do you prepare a % NaCl solution? Tell whether the medium is...
Post-Lab Questions For each of the tubing pieces, identify whether the solution inside was hypotonic, hypertonic,...
Post-Lab Questions For each of the tubing pieces, identify whether the solution inside was hypotonic, hypertonic, or isotonic in comparison to the beaker solution in which it was placed. Which tubing increased the most in volume? Explain why this happened. What do the results of this experiment this tell you about the relative tonicity between the contents of the tubing and the solution in the beaker? What would happen if the tubing with the yellow band was placed in a...
Lab 9 Review Name 8 12x4 Why do you think that some your TSA plate? Date...
Lab 9 Review Name 8 12x4 Why do you think that some your TSA plate? Date microorganisms present in/on the location you sampled did not grow on Section 12,800X 9 What are biofilms and how are they formed in nature? Give examples of biofilms. fthe 12 0 the 2 10 Imagine that you have an agar plate with 3 isolated colonies formed by bacteria A, B, C and with generation times of 30, 60 and 120 minutes, respectively (generation time...
3. The bacterium Corynebacterium glutamicum has been aerobically cultured in a batch fermenter vessel on glucose...
3. The bacterium Corynebacterium glutamicum has been aerobically cultured in a batch fermenter vessel on glucose at pH 7.0 and 20 °C to produce Glutamic acid for the manufacture of Mono Sodium Glutamate, a flavour compound for the food industry. The time course data for a typical batch culture of this bacterium in a fermenter vessel are given below: t (h) Cell dry weight (g l-1) Glucose concentration (g l-1) Glutamic acid concentration (g l-1) 0 1.22 100 0 8 ...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4....
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4. For what purpose do bacteria that have the enzyme utilize it? 6. What will occur if the plate is incubated right side up? 7. What will occur if the plate is incubated for more than 24-48 hours? Exercise 7: Selective and Differential Growth Medium and Tests 95 Exercise 7.9 Hydrolytic Exoenzymes: Lipase and Casease LIPASE TEST Lipids are fatty acids used by some bacterium...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
You notice that there is an increasing prevalence of a bacterial infection at your hospital that...
You notice that there is an increasing prevalence of a bacterial infection at your hospital that appears to be affecting people. The bacterium has been named T. routmania, and microbiologists have found that it is a gram negative organism that is highly resistant to typical antibiotics. Immunologists have found that the organism appears to target T-cells in the host. Analysis of lymphatic fluid from infected patients has found an unusually low level of IL-2, yet tissue samples appear to have...
Please need help answering question A the pages of background information are posted thanks Read page...
Please need help answering question A the pages of background
information are posted thanks
Read page 196-197 and figure 6.20. regarding Meselson and
Stahl’s experiment regarding DNA replication. And Answer the
following question
If you are using this radioactive technique in mouse cells,
what would happen in each phase of G1, S, G2, mitosis and meiosis
assuming that you are grown cells in 15N medium for many
generations and cells in G1are then switched to 14N medium?
G1
S
G2...
CRITICAL THINKING QUESTIONS 1. A pre-weighed dialysis bag which contained a solution of 10% glucose was...
CRITICAL THINKING QUESTIONS 1. A pre-weighed dialysis bag which contained a solution of 10% glucose was placed in a beaker containing a solution of 20% glucose. After one hour, the bag was weighed again a. Calculate the % mass change of this dialysis bag from the following information: • Mass of bag before experiment: 15.39 • Mass of bag after experiment: 12.79 b. Determine the tonicity of the beaker when it is compared to the dialysis bag: 2. facell had...
Lab 9 Review Name 8 12x4 Why do you think that some your TSA plate? Date microorganisms present in/on the location you sampled did not grow on Section 12,800X 9 What are biofilms and how are they formed in nature? Give examples of biofilms. fthe 12 0 the 2 10 Imagine that you have an agar plate with 3 isolated colonies formed by bacteria A, B, C and with generation times of 30, 60 and 120 minutes, respectively (generation time...
LAB17 Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
2. What is the enzyme being tested? 3. What is the substrate if the enzyme? 4. For what purpose do bacteria that have the enzyme utilize it? 6. What will occur if the plate is incubated right side up? 7. What will occur if the plate is incubated for more than 24-48 hours? Exercise 7: Selective and Differential Growth Medium and Tests 95 Exercise 7.9 Hydrolytic Exoenzymes: Lipase and Casease LIPASE TEST Lipids are fatty acids used by some bacterium...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...
LAB Genetic Engineering of Bacteria Problem Is it possible to transfer the allele for resistance to the antibiotic ampicillin into a bacterial cell? Objectives After completing this lab, the student will be able to: 1. Demonstrate micropipetting and sterile pipetting techniques for handling and transferring bacteria and plasmid DNA. 2. Maintain sterile conditions for culturing bacterial cells. 3. Inoculate bacteria into flasks, culture tubes, or agar plates. 4. Culture isolated individual colonies from an agar plate to form genetically identical...
Please need help answering question A the pages of background
information are posted thanks
Read page 196-197 and figure 6.20. regarding Meselson and
Stahl’s experiment regarding DNA replication. And Answer the
following question
If you are using this radioactive technique in mouse cells,
what would happen in each phase of G1, S, G2, mitosis and meiosis
assuming that you are grown cells in 15N medium for many
generations and cells in G1are then switched to 14N medium?
G1
S
G2...
CRITICAL THINKING QUESTIONS 1. A pre-weighed dialysis bag which contained a solution of 10% glucose was placed in a beaker containing a solution of 20% glucose. After one hour, the bag was weighed again a. Calculate the % mass change of this dialysis bag from the following information: • Mass of bag before experiment: 15.39 • Mass of bag after experiment: 12.79 b. Determine the tonicity of the beaker when it is compared to the dialysis bag: 2. facell had...
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