 V (umol S / mlsec) 0.61 1.19 2.78 5.00 8.33 13.9 2 10 20 50 What is Vmax?](http://img.homeworklib.com/questions/1b1b4050-c49b-11eb-a9c8-79e6c504b2ab.png?x-oss-process=image/resize,w_560)
Also, the question asks for the
Km
Homework Answers
![[S] (mM) V (mmol S/ml sec 1/Is 0.00061 0.00119 0.00278 0.005 0.00833 0.0139 1639.344262 840.3361345 359.7122302 200 120.04801](http://img.homeworklib.com/questions/4064e7d0-c49b-11eb-870b-b176fae3b952.png?x-oss-process=image/resize,w_560)
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Also, the question asks for the
Km
Experimental values S](mM) V (umol S / ml'sec) 0.61...
a. An enzyme has a Vmax of 100 umol/min and a Km of 40 uM. When...
a. An enzyme has a Vmax of 100 umol/min and a Km of 40 uM. When substrate concentration is 40 uM what is the initial reaction rate? b. An enzyme with a Vmax of 100 umol/minute and a Km of 10 uM was reacted with a irreversible active site specific inhibitor. After reaction with the inhibitor, the enzyme was assayed using a 2 mM concentration of substrate, and it gave a reaction rate of 20 umol/min. What percentage of the...
Exp normal inhib1 inhib2 [S](mM) VV V(all units are umol/(mL'sec)) 2 24 Assuming [Etotal] is the...
Exp normal inhib1 inhib2 [S](mM) VV V(all units are umol/(mL'sec)) 2 24 Assuming [Etotal] is the same in all experiments. [ 5 mM for both cases. What is Vmax and Km of the enzyme? What type of Inhibitor is inhib1 and inhib2? What is the Kl of inhib1 and inhib2? 24 8.6 11 80 52 36
Nobody has been able to solve the Km. The Vmax values are correct. I believe I...
Nobody has been able to solve the Km. The Vmax values are
correct. I believe I have my graph wrong. Can someone please help
me solve this.
Using the Lineweaver-Burk Equation 1/Vo Km/Vmax[S]1/Vmax) create a graph of both the Non-Inhibited data and Inhibited data below (on the same graph axes) and calculate the KM and VMax for each line. Vo (umol/L.min) Vo (Hmol/L-min) [Sol (umol/L) Non-Inhibited Data Inhibited Data .00e-06 13.9 7.60 .00e-06 18.0 9.90 ..10e- 05 10e-05 26.0 14.8...
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol...
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol ml-Imin-1) 0.1 0.2 5.00 7.14 8.0 1.0 8.33 9.09 (a) From a double-reciprocal plot of the data, determine Km and Vmax. (b) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number 0.8 2.0
Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S] (mM) V (mmol ml-1min-1)...
Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S] (mM) V (mmol ml-1min-1) 0.1 3.33 0.2 5.00 0.5 7.14 0.8 8.0 1.0 8.33 2.0 9.09 a.) From a double-reciprocal plot of the data, determine Km and Vmax. b.) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number.
Find Km, Vmax, Ki and Kcat values. Find Vmax, Km and Ki values according to the...
Find Km, Vmax, Ki and Kcat values.
Find Vmax, Km and Ki values according to the graph
350 y = 51.843x + 99.634 300 y = 45.555x + 93.809 250 y = 33.086x + 88.634 200 No In 150 100 10 pl • 20 ul ........ Linea -50 Linea -100 Benze Linea 1/[S] mM Benze
11. In Excel, prepare Lineweaver-Burk plots for the behavior of an enzyme for which the following...
11. In Excel, prepare Lineweaver-Burk plots for the behavior of an enzyme for which the following experimental data are available: V, umol/min umol/min (No Inhibitor) S], mM (Inhibitor Present) 3.66 5.12 6.18 6.98 7.60 4.58 6.40 7.72 8.72 9.50 3.0 5.0 7.0 9.0 11.0 a. What are the KM and Vmax values for the inhibited and uninhibited reaction 5 pts. each reaction) b. Is the inhibitor competitive or noncompetitive? (5 pts.) Micheli-Menten) EQUATIONS: VV
Use the values from Table 13 to generate a Table showing 1/[S] and 1/V Plot Lineweaver...
Use the values from Table 13 to generate a Table showing 1/[S]
and 1/V Plot Lineweaver Burke (1/v vs. 1/[s]) and caculate Km and
Vmax in the absence and presence of 1mM inhibitor A. What is the
nature of inhibitor A?
Plot 1/V vs 1/[S], and caculate the values of Km and Vmax in the
absence and presence of 3 mM and 5mM of inhibitor B. What is the
nature of inhibitor B?
Plot 1/V vs [I] i .e Dixon...
a. what are the values of Vmax and Km in the abscence if the inhibitor what...
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
Kcat =30.0 s-1 Km= 0.005 M Operating at 1/4 Vmax What is [S] ? The solutions...
Kcat =30.0 s-1
Km= 0.005 M
Operating at 1/4 Vmax
What is [S] ?
The solutions manuel doesn't explain the problem well. Whete
does the 0.33 km come from?
For part 2 : Plug in [S]= 1/2 Km, 2 Km, and 10 Km
Where does the 1.5 Km come from?
Answer (a) Here we want to find the value of [S] when Vo = 0.25 V max. The Michaelis-Menten equation is V = Vmax[S]/(Km + [SD so V = Vmax...
Exp normal inhib1 inhib2 [S](mM) VV V(all units are umol/(mL'sec)) 2 24 Assuming [Etotal] is the same in all experiments. [ 5 mM for both cases. What is Vmax and Km of the enzyme? What type of Inhibitor is inhib1 and inhib2? What is the Kl of inhib1 and inhib2? 24 8.6 11 80 52 36
Nobody has been able to solve the Km. The Vmax values are
correct. I believe I have my graph wrong. Can someone please help
me solve this.
Using the Lineweaver-Burk Equation 1/Vo Km/Vmax[S]1/Vmax) create a graph of both the Non-Inhibited data and Inhibited data below (on the same graph axes) and calculate the KM and VMax for each line. Vo (umol/L.min) Vo (Hmol/L-min) [Sol (umol/L) Non-Inhibited Data Inhibited Data .00e-06 13.9 7.60 .00e-06 18.0 9.90 ..10e- 05 10e-05 26.0 14.8...
3.33 3. Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S(mm) V (mmol ml-Imin-1) 0.1 0.2 5.00 7.14 8.0 1.0 8.33 9.09 (a) From a double-reciprocal plot of the data, determine Km and Vmax. (b) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number 0.8 2.0
Find Km, Vmax, Ki and Kcat values.
Find Vmax, Km and Ki values according to the graph
350 y = 51.843x + 99.634 300 y = 45.555x + 93.809 250 y = 33.086x + 88.634 200 No In 150 100 10 pl • 20 ul ........ Linea -50 Linea -100 Benze Linea 1/[S] mM Benze
11. In Excel, prepare Lineweaver-Burk plots for the behavior of an enzyme for which the following experimental data are available: V, umol/min umol/min (No Inhibitor) S], mM (Inhibitor Present) 3.66 5.12 6.18 6.98 7.60 4.58 6.40 7.72 8.72 9.50 3.0 5.0 7.0 9.0 11.0 a. What are the KM and Vmax values for the inhibited and uninhibited reaction 5 pts. each reaction) b. Is the inhibitor competitive or noncompetitive? (5 pts.) Micheli-Menten) EQUATIONS: VV
Use the values from Table 13 to generate a Table showing 1/[S]
and 1/V Plot Lineweaver Burke (1/v vs. 1/[s]) and caculate Km and
Vmax in the absence and presence of 1mM inhibitor A. What is the
nature of inhibitor A?
Plot 1/V vs 1/[S], and caculate the values of Km and Vmax in the
absence and presence of 3 mM and 5mM of inhibitor B. What is the
nature of inhibitor B?
Plot 1/V vs [I] i .e Dixon...
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
Kcat =30.0 s-1
Km= 0.005 M
Operating at 1/4 Vmax
What is [S] ?
The solutions manuel doesn't explain the problem well. Whete
does the 0.33 km come from?
For part 2 : Plug in [S]= 1/2 Km, 2 Km, and 10 Km
Where does the 1.5 Km come from?
Answer (a) Here we want to find the value of [S] when Vo = 0.25 V max. The Michaelis-Menten equation is V = Vmax[S]/(Km + [SD so V = Vmax...
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