Question

Discussion 1. Is there any difference in staining between the nucleus and cytoplasm? Why? 2. Is the polysaccharide homogeneously distributed throughout the cytoplasm or is it granular in appearance? Why? 3. Monosaccharides such as glucose are used in energy metabolism. Would they obscure glycogen staining in the slide you prepared? Explain your answer.

Answer 1

1.

Not only nucleus and cytoplasm, but there are differences between the staining of different cell organelles. This is because of the differences in composition of these organelles.

Nucleus stains differently from cytoplasm. This is because nucleus contains the genetic material DNA which is associated with histone proteins. It is a compactly arranged system of DNA and proteins. Therefore it appears a darkly stained body inside the cell.

On the other hand cytoplasm is a watery fluid into which all the cell organelles are suspended. It is present throughout the cell and is not as compact as the nucleus. Therefore it gives a granular appearance after staining. The granules represent different cell organelles. It is stained very lightly in comparison to Nucleus.

2.

Polysaccharides are not homogenously distributed in the cytoplasm. They are restricted to certain locations inside the cell. For example a structural polysaccharide like cellulose will be seen only in the cell wall of the plant cells where as a storage polysaccharide like starch will be seen as starch granules inside the cytoplasm. This will not give a homogenous appearance to polysaccharides. Non-homogenous appearance is attributed to their restricted distribution.

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