ZuoTi.Pro
Question

In the PCR exercise you described why we amplify the 16s ribosomal RNA gene. What other...

In the PCR exercise you described why we amplify the 16s ribosomal RNA gene. What other genes might help us to identify specific bacteria? In other words, what traits could be sequenced that are unique to bacteria?

science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

Genes encoding specific enzymes in bacteria can be used for the identification of species of bacteria.

Some of the genes encoding for following enzymes can be used -

Restriction enzymes

Catalase

Enzymes involved in nitrogen fixation

Lactose metabolism

Inulin fermentation

Raffinose fermentation

Genes encoding for specific antibiotics

Out of all the listed genes, the most specific will be genes encoding for antibiotics and restriction enzymes. Although, all of them can be used for the identification of a species of bacteria by PCR.

Please give a good rating.

0 0
Add a comment
Know the answer?
Add Answer to:
In the PCR exercise you described why we amplify the 16s ribosomal RNA gene. What other...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • In the protocol we followed, 16S rRNA gene segments were amplified by PCR, then individually ligated...

    In the protocol we followed, 16S rRNA gene segments were amplified by PCR, then individually ligated into a vector, transformed and propagated in bacteria, and 9 bacterial “clones” sequenced by the Sanger method. Each clone had a unique sequence we subsequently compared. If we had used an Illumina Next Generation Sequencing method, would the “cloning” of individual fragments have been necessary?

  • What would the correct answer for this question be and why? And why would the other...

    What would the correct answer for this question be and why? And why would the other choices be wrong? We were unable to transcribe this imageQuestion 85 Which of the following would most strongly reduce the strength of a real-time colony PCR? O A. 16S rRNA has minor SNPs between different species of bacteria. O B. Consistently running the PCR amplification for one O C. Different species of bacteria have different numbers O D. Different species of bacteria express 16S...

  • Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This...

    Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This is a lab we did and some homework questions regarding the sequencing rDNA to find unknown bacteria. I hope by answering these I can have better understanding of the process. The more descriptive the better. Thank you! 1. A. Name the gene that we will be sequencing in order to identify the unknown. Explain why this region is considered to be the target for...

  • You are using PCR to amplify a 300 bp target sequence, a portion of Gene X,...

    You are using PCR to amplify a 300 bp target sequence, a portion of Gene X, from human genomic DNA isolated from patients' blood samples. The instructions for this procedure tell you to include Samples A and B, whose contents are listed below, with each batch of patient samples that you run. Ingredients Sample A Sample B 10x PCR Buffer (Tris,KCI,MgCl2,BSA) 5 mL 5 mL H2O 37.8mL 38.8mL dNTP's 3 mL 3 mL Taq DNA polymerase 0.2 mL 0.2 mL...

  • Please have a different answer than the other post, and use layman's terms as well hhmi...

    Please have a different answer than the other post, and use layman's terms as well hhmi Biolnteractive Using CRISPR to identify the Functions of Butterfly Genes Activity Student Handout This document is made available by the Howard Hughes Medical Institute. Using this document, you agree to use this document in accordance with the Terms of Use. INTRODUCTION Scientists have determined the complete DNA sequences of the genomes for many organisms, including humans. By analyzing patterns in those sequences, they can...

  • Why do we want/need the presence of Mg2+ in a PCR but do not want it...

    Why do we want/need the presence of Mg2+ in a PCR but do not want it present at all other steps of processing/analyzing a DNA sample? (2 marks) Identify and explain what generally occurs in each of the 3 main steps of a polymerase chain reaction. (6 marks) 8. Identify and explain what the 3 different PCR controls inform you about the results of a PCR. (3 marks) Highlight the 2 advantages and 3 disadvantages of using PCR. Ensure to...

  • 1. The following is a sequence of RNA. Do you expect it to adopt any kind...

    1. The following is a sequence of RNA. Do you expect it to adopt any kind of structure, and, if so, show what the structure would be? 5'-AUACUGCCCCCGGGGGAUGCCCGUAAACCGGGGUUACCCGGUUUAAACGGCCCCCCGGGGGCAGGCG-3'. 2. A dipoid yeast was ABD on one chromosome and abd on the other. The yeast cell underwent meiosis and the four genomes in the resulting tetrad were analyzed and determined to be: ABD, ABD, ABD and abd. a. For the moment, focus on the outcome regarding the number of B versus...

  • 1-. List three "big questions" you have about the solid Earth or Earth Process. An example...

    1-. List three "big questions" you have about the solid Earth or Earth Process. An example would be "Have the Himalaya mountains always been this tall and why" or "Why do we have so many Earthquakes every day".  Note: This is a physical geology course, keep your questions relevant.  In other words, the physical world and natural processes we live in. NO Biology questions about bacteria, Yetis, llamas, frogs, etc. 2-. What are some ways that you could start to find the answers, and HOW exactly would...

  • QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...

    QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted    QUESTION 2: You are performing a PCR using primers with a sequence perfectly...

  • Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...

    Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT