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Question 4 2 pts You counted 200 cells in one "red" ("A", "big", 1 mm x...
200 In making a leukocyte count, a 1:20 dilution is made. The number of cells counted...
200 In making a leukocyte count, a 1:20 dilution is made. The number of cells counted in the corner squares of the counting chamber is 200. What is the total leukocyte count? dilution -200 200 LUIS X70 x 20 Avoneze= Depth:10 4 4 2500 - 50 TOX T Totul leukocyte- count 2.5xius ue in making a leukocyte count, the blood is diluted to 1:50 and the number of cells counted in the 8 WBC corners on both sides of the...
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrie...
#1-6
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count...
help answer all the answer with the solution thank you. 1. Given the following values, calculate...
help answer all the answer with the solution thank you.
1. Given the following values, calculate the RPI: observed reticulocyte count=6.0%;Het = 0.30 L/L (which would give a maturation time of 2.0 days) 2. A manual white blood cell count was performed. A total of 36 cells were counted in all 9 "larger" squares of a Neubauer-ruled hemacytometer. A 1:10 dilution was used. What is the WBC? 3 3. In a reticulocyte count, 985 red cells and 15 reticulocytes were...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
You will be plating 80,000 cells each into two wells on your chamber slide. Each well...
You will be plating 80,000 cells each into two wells on your chamber slide. Each well takes 1 ml of media. Your cell solution has a concentration of 2 x 106 cells/ml. By what dilution factor will you need to dilution the cell solution?
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with...
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
1. Why do you dilute the fibroblasts in trypan blue before counting them? What will the trypan blue...
1. Why do you dilute the fibroblasts in trypan blue before counting them? What will the trypan blue tell you about the cells? 2. Why do you add trypsin to the fibroblasts in the culture dish? How does trypsin do this? 3. You have counted the 4 corner squares of a hemocytometer grid (each square is 1mm2 and the distance of the coverslip above the squares is 0.1mm) and have come up with a total cell count of 243 cells. What is your original...
1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an...
1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an aliquot of the cell suspension and counted the cells using a hemocytometer. There were 75 cells in the 16 little squares. Please explain how to get each! a. Calculate the concentration of the cells prior to the dilution. Show your calculations. Use scientific notation to express the result. b. Now, you want to seed a 25cm2 flask with 750,000 cells. Calculate how many ml...
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4 cells/mL and need to produce a solution of 56 cells/mL set up detailed serial dilution given 8mL of 4.1 x10^3 cells/ML and need approximitly 100 cells/mL. set up detailed serial dilution by determining the aliquot to be transfered (v1) from the solution to get 100 cells/mL in a final volume of 10 mL given 10mL cell suspension of NIH cells at 3.8 x10^5 cells/ML...
1) The approximate cell yield of a T25 flask (for adherent cells) is 2.8 x 106...
1) The approximate cell yield of a T25 flask (for adherent cells) is 2.8 x 106 cells. The cell yield is the total amount of adherent cells at confluence. How many adherent cells would you expect to be in this flask at 80% confluence? 2) For adherent cells, the seeding density is usually between what range of % confluencies? 3) How many total cells would you place in a T75 flask with a cell yield of 8.4 x 106 cells...
200 In making a leukocyte count, a 1:20 dilution is made. The number of cells counted in the corner squares of the counting chamber is 200. What is the total leukocyte count? dilution -200 200 LUIS X70 x 20 Avoneze= Depth:10 4 4 2500 - 50 TOX T Totul leukocyte- count 2.5xius ue in making a leukocyte count, the blood is diluted to 1:50 and the number of cells counted in the 8 WBC corners on both sides of the...
#1-6
1. Calculate the D.F for a bacterial culture if 2000uL of undiluted cuiture was added to a nutrient broth to bring up the final volume to 1 liter. (2 pts) a. 102 b. 2 X 10 c. 2 X 104 2. It you plated 20 ul of sample with a dilution factor of 10-3, what would your total sample volume be? (Total sample volume - (Volume plated) x (Dilution)). (2 pts) 3. Calculate the O.C.D using a viable count...
help answer all the answer with the solution thank you.
1. Given the following values, calculate the RPI: observed reticulocyte count=6.0%;Het = 0.30 L/L (which would give a maturation time of 2.0 days) 2. A manual white blood cell count was performed. A total of 36 cells were counted in all 9 "larger" squares of a Neubauer-ruled hemacytometer. A 1:10 dilution was used. What is the WBC? 3 3. In a reticulocyte count, 985 red cells and 15 reticulocytes were...
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