you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...

Question

Question

you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...

you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4 cells/mL and need to produce a solution of 56 cells/mL set up detailed serial dilution

given 8mL of 4.1 x10^3 cells/ML and need approximitly 100 cells/mL. set up detailed serial dilution by determining the aliquot to be transfered (v1) from the solution to get 100 cells/mL in a final volume of 10 mL

given 10mL cell suspension of NIH cells at 3.8 x10^5 cells/ML set up detailed dilution series in detail so that you plate 1 mL and have exactly 100 cells/mL final

science biology

Add a comment Improve this question Transcribed image text

Answer 1

Answer #1

Add a comment

Answer 2

Similar Homework Help Questions

1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an...

1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an aliquot of the cell suspension and counted the cells using a hemocytometer. There were 75 cells in the 16 little squares. Please explain how to get each! a. Calculate the concentration of the cells prior to the dilution. Show your calculations. Use scientific notation to express the result. b. Now, you want to seed a 25cm2 flask with 750,000 cells. Calculate how many ml...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...

You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...

1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...

Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....

Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
my questions are the following: A) what is the concentration of the stock solution of LDH?...

my questions are the following: A) what is the concentration of the stock solution of LDH? B) Based upon Part B of the protocol, show your calculations to make the 250ug/ml LDH solution. C) Based upon Part B of the protocol, show your calculations to make the 25ug/ml LDH solution. You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...

Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...

You only have 150 pl stock solution that contains 100 mgml of protein. You want to...

You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...

please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification...

Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification of LeuRS F C Terminal Domain. I am having troubles figuring out the rest of my caluclations. I already did the calculations for the first dilution set, I was wondering how I would do the calculation for the second dilution set. Also I was wondering how I would determine the theoretical values for my data. Is there a specific formula I can use? If...

PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone,...

PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone, 0.5% yeast extract, 10 mM NaCL (F.W. 58), 2.5 mM KCl (F.W. 74.5), 10 mM MgSO4 (F.W. 246.5), 10 mM MgCl2 (F.W. 203.3), 20 mM glucose (F.W. 180.16). Determine the amounts of each to make 500 mL of SOC. 2) Determine the number of moles in 0.05 μg of 300 bp insert DNA. There are 660 g/mol-bp. What is the molar ration of insert...

you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...

Homework Answers

Add Answer to:
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...

Post as a guest

Earn Coins

1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an...

You have a stock solution that contains 24 mg/ml of protein. You want to make a...

1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...

Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....

my questions are the following: A) what is the concentration of the stock solution of LDH?...

Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...

You only have 150 pl stock solution that contains 100 mgml of protein. You want to...

Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...

Hi I am im a biochemistry lab and we are doing an experiment titled: PCR Amplification...

PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone,...

you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...

Homework Answers

Add Answer to: you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...

Post as a guest

Earn Coins

Add Answer to:
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4...