Question

1) Defing whx a SNP is. Whas does SNP suad for and what type of mutalion does 2) Whaa does a restrictioes endomle doeng 3) Explain why the MISTII restrictie mionuclease cuts the lhAくwild type) pene·ed rot the Hbs (mutant type, sicklecell gme 4) Describe the genotope for a patient who eshilbits the sickle cell phesotype Describe the sickle cell phenotype and potential consequences of someone who hs i. 5) How many fragments of DNA are created it the MST II restriction eneyme is treated on the following senotypes: HbAlh, HSHhs, and HbA/Hbs 6) If there are four restriction sites (areas where restriction endonucleases act) on a strand of DNA, how many bands we would expect to see on a gel? 7) Explain the major steps of gel electrophoresis. Why dkes DNA run from the negdive electrode to the positive electrode? 8) Describe the six steps necessary to be successtiul in operating a micrupipette

I don't understand the rest, please help.

Answer 1

1. SNP stands for single nucleotide polymorphism. It is the variation in single nucleotide occurring at the specific position in genome. This causes cystic fibrosis and sickening cell anemia.

2. Restriction endonucleases are the enzymes that cleaves DNA at specific sites. Such sites where these enzymes cleave are called as restriction sites .