Question

Gradient elution in ion-exchange chromatography may involve:_________. A) changing the polarity of the eluent. B) increasing...

Gradient elution in ion-exchange chromatography may involve:_________.

A) changing the polarity of the eluent.

B) increasing the temperature of the system.

C) increasing the ionic strength of the eluent.

D) modifying the rate flow of the eluent.

E) continuously increasing the pressure.

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Answer #1

Gradient ellusion of ion-exchange chromatography may involve increasing the ionic strength of the eluent.

This is because in gradient ellusion of ion exchange chromatography varying the concentration of the eluent ions with widely differing affinity for the seprator resin can be elute in one run. We also know that with the help of concentration,ionic strength will be measure.

So option (C) is the correct answer.

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