Homework Answers
Ion exchange chromatography is based on the (A) electrostatic attraction.
The principle of this chromatography is that electrostatic attraction binds charged molecules (say negatively charged protein) with the oppositely charged stationary phase (eg. positively charged matrix).
Following the binding event, ionic strength is altered to
exchange the bound molecules with ions having a similar
charge.
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5) Ion exchange chromatography is based on the A) electrostatic attraction B) electrical mobility of ionic...
Gradient elution in ion-exchange chromatography may involve:_________. A) changing the polarity of the eluent. B) increasing...
Gradient elution in ion-exchange chromatography may involve:_________. A) changing the polarity of the eluent. B) increasing the temperature of the system. C) increasing the ionic strength of the eluent. D) modifying the rate flow of the eluent. E) continuously increasing the pressure.
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pKa is 6.0). If I wanted to seperate protein X, whose pl is 3.0x what is the pH range of the buffer that can be used for ion exchange chromatography with this resin ? A. any pH between 3.0 and 6.0 B. there is no pH range for this resin and protein X C. any pH above 4.0 D. any pH below 6.0
In cation exchange chromatography, the beads are covalently linked to a ________chemical and a ______________ ion...
In cation exchange chromatography, the beads are covalently linked to a ________chemical and a ______________ ion is used as the counter-ion. a. positive, negative b. negative, positive c. negative, negative d. positive, positive Which of the following is NOT an application of a DNA microarray? a. SNP detection b. comparative genomic hybridization c. gene expression profiling d. alternative splicing detection e. imprinting Individuals susceptible to tumorigenesis have an abundance of receptors specific for: a. serotonin b. testosterone c. acetylcholine d....
2- ECD 3- ICP-AES 4- TCD 5- AAS 6- GLC 7- SHE 8- SEC Q4- What...
2- ECD 3- ICP-AES 4- TCD 5- AAS 6- GLC 7- SHE 8- SEC Q4- What kind of chromatography wil be most applicable to separate the following substances: (choose from the following: gas- liquid chromatography, liquid-solid partition chromatography, reversed phase partition chromatography, adsorption chromatography, ion-exchange chromatography, gel filtration chromatography, gel permeation chromatography) (3.5 points) Chromatographic separation technique Mixture a- Gasoline fuel components (mostly a mixture of aliphatic hydrocarbon compounds) b- Free amino acids (*NH3-CHR-COO) (Analine, Leucine, Valine, aspartic acid, Glycine...
Chromatography Basics Multiple Choice: Answer the following multiple choice questions about Chromatography Basics (SHOW WORKING): 1) In...
Chromatography Basics Multiple Choice: Answer the following multiple choice questions about Chromatography Basics (SHOW WORKING): 1) In a given chromatographic column, A) different solutes behave as if there are a different number of theoretical plates for each. B) the number of theoretical plates is the same for all solutes. C) efficiency is improved by increasing plate height. 2) What useful information can be found from a Van Deemter plot? A) The selectivity factor B) Optimum mobile phase flow rate C) Optimum...
a) Providing an expression, estimate the electrical potential energy of a Potassium ion and a Chlorine...
a) Providing an expression, estimate the electrical potential energy of a Potassium ion and a Chlorine ion separated by a distance of 0.36nm b) State at least one important assumption explicit to your expression. c) For ionic molecules, provide an expression that defines the relationship between the electric potential energy and the bond disassociation energy. d) From the information provided above, justifying your answers with fundamental arguments, what would you expect for i) the electrical potential energy and ii) the...
A Lewis structure of the carbamate ion is shown. :0: H. H Lewis structure A Draw...
A Lewis structure of the carbamate ion is shown. :0: H. H Lewis structure A Draw the two other major resonance structures. Be sure to include all nonbonding electrons and formal charges where appropriate, Lewis structure C Lewis structure B More Rings Select Draw Erase Rings More Select Draw H C z Question 1 of9 The charges and sizes of the ions in an ionic compound affect the strength of the electrostatic attraction holding that compound together. Based on ion...
Q10. Highlight in the following colours the correct descriptions of: metallic bonds, covalent bonds and ionic...
Q10. Highlight in the following colours the correct descriptions of: metallic bonds, covalent bonds and ionic bonds A. Sharing of valence electrons B. Magnetic dipole forces C. Repulsion between ions being shielded by a valence electron cloud D. Exchange of an electron between two atoms E. Electrostatic attraction between a proton and the electron cloud of a F atom
An ion exchange resin is used to remove nitrate from a water supply with the ionic...
An ion exchange resin is used to remove nitrate from a water supply with the ionic concentrations found below. The total resin capacity is 1.5 equivalents per liter of resin. a) Do the anions and cations balance? b) What volume of water can be treated with each liter of resin? c) Qualitatively, how would your answer differ if the concentration of CI^- and SO_4^2- were reversed?
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
2- ECD 3- ICP-AES 4- TCD 5- AAS 6- GLC 7- SHE 8- SEC Q4- What kind of chromatography wil be most applicable to separate the following substances: (choose from the following: gas- liquid chromatography, liquid-solid partition chromatography, reversed phase partition chromatography, adsorption chromatography, ion-exchange chromatography, gel filtration chromatography, gel permeation chromatography) (3.5 points) Chromatographic separation technique Mixture a- Gasoline fuel components (mostly a mixture of aliphatic hydrocarbon compounds) b- Free amino acids (*NH3-CHR-COO) (Analine, Leucine, Valine, aspartic acid, Glycine...
A Lewis structure of the carbamate ion is shown. :0: H. H Lewis structure A Draw the two other major resonance structures. Be sure to include all nonbonding electrons and formal charges where appropriate, Lewis structure C Lewis structure B More Rings Select Draw Erase Rings More Select Draw H C z Question 1 of9 The charges and sizes of the ions in an ionic compound affect the strength of the electrostatic attraction holding that compound together. Based on ion...
An ion exchange resin is used to remove nitrate from a water supply with the ionic concentrations found below. The total resin capacity is 1.5 equivalents per liter of resin. a) Do the anions and cations balance? b) What volume of water can be treated with each liter of resin? c) Qualitatively, how would your answer differ if the concentration of CI^- and SO_4^2- were reversed?
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
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