Question

5. Based on the binding curves of different versions of myoglobin shown below: -Myoglobin type 1 -Myoglobin type 2 20 30 40 70 80 10g 50 60 PO, (torr) a. What are the pSos for the curves? (3 pts) b. Which type of Myoglobin binds oxygen better? Explain. (4 pts)

Answer 1

For a myoglobin protein and O₂ ligand equilibrio crill be P+ L € PL P= Protein La Ligand Now anociation constant kat [PL] [p] [L] And dissociation constant id= IP] [L) - [PL] And o inc. fraction occupied by lizard binding sites occupied total sinding siten . [PL] [PL]+[P] EL) (Putting, [PL] = kapal IL) it ka

Hoca as O in ganeons in so the equation and become - pq + + At o = 0.5-5. - AA N . Þ O + + . 1 = p at a = o.s ewe which is called pso

For Myoglotain type - 1 At a=0.5, 102 = 20 tora. from the graph. tonn. so pso = 20 For Myoglobin type-2 I from the A A so a=0.5, 10= 40 ton pso = 40 dona. Myoglobin binds a Sollon PAL P2 . better. so it will IPL) , kao ip] [2) this equilibrium. so in arhich this ka equilibrium constant chill be higher that auall bind better We can also say in the other cray around in anhich I value in lesser that arill binda better. How I = p 5o. As Myoglobin. Aype-2 hen lessen pso nie. 20 korn an.rot. Myoglobina tape-2 arbach han 40 kon. so Myoglobin type- 1 birds better to O₂.