Question

biochemistry need help!

1. Answer the following series of questions related to the curve shown below for Hemoglobin (Hb) and Myoglobin (Mb) binding to oxygen 1.00 p.so 0.60 0.40 0.20 0.0 C 120 80 100 60 20 40 What is the implication of the differences between the points A and C on the same binding curve? a. (ie. what does this reflect?) whertcs point 30 (condntin ofL) Pont A reaches 507 5oturaien o sleur Hd thar is a hihr Ka. Wth pok A havin hes a cmach What is the implication of the differences between the points A and B on the different binding b. curves? (i.e. what does this reflect?) . For the points A and D, something is the same about them - what is it and what does it mean? (2) c. For the points A and D, something is different about them - what is it and use it to identify which point belongs to Mb and which to Hb. d. living in Colorado (i.e. high altitude). Describe the How would the "C" point shift if you were implications of this. e. How would the "A" point shift if you increased synthesis of BPG. Describe the importance of this. f. 1

Answer 1

b. The point B is falling on the curve that showing myoglobin and the point A is falling on the hemoglobin. The difference between the two is B is showing that myoglobin have high affinity for oxygen, therefore, saturated with oxygen after high taking of oxygen whereas point A is showing that hemoglobin get saturated with oxygen very fast.

c. Point A and D is showing the oxygen content when hemoglobin and myoglobin get saturated with half of the available oxygen.

d. Point D belong to myoglobin and point A belong to hemoglobin. Both point differ in the the partial pressure of oxygen. Myoglobin get half saturated with oxygen when partial pressure of oxygen is low whereas hemoglobin get saturated with oxygen when partial pressure of oxygen is high.

e. At high altitudes, the patial pressure of oxygen get reduced and hence, more hemoglobin will bind to the oxygen, that change the location of C towards left.

f. BPG decreases the activity of hemoglobin for the oxygen. Therefore, if synthesis of BPG get increased, the oxygen dissociation curve shift towards right.