Question

please use these results of a growth curve experiment to answer the following questions

Questions 1) Plot the OD600 readings and cell concentration for each time point on a scatterplot with a line connecting the points. Have the measured data (OD600 and cell concentration) on the y-axis against the time on the x-axis using Excel (or other computer based data/graphing program). It is helpful to set the y-axis on a log scale for the cell concentration measurements. 2) Can you recognize the different phases of the growth curve in your plot of the data? Label them on the graph. If not, what are possible reasons for difference in the growth curve you observed vs, what is expected? 3) Use the logarithmic phase of the growth curve to estimate the generation time (g units are hours/generation; also known as the doubling time) of E. coli. How does the generation time that you estimated compare to the published generation times for E. coli growing under optimal conditions? What are some of the possible reasons for differences in the generation times? What are possible sources of error in this exercise? 4) Plot the cell concentration (X-axis) vs. OD 600 (y-axis). Determine the standard curve of your culture by fitting a regression line to the points or calculating the average slope between points. What is the future benefit of knowing the standard curve for this culture and being able to predict the cell concentration for any OD600?

Answer 1

2.0- cell conc OD x 10' cells/ml OD * 0.5- 0.0 ned Samscannte Time