Homework Answers
You can choose any two values
from the above table to find out the number of generations and then
generation time. We calculated 2.6 minutes of generation time
here.
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OBSERVATIONS AND RESULTS 1. Record your results of absorbance values at 600 nm and subsequently plot...
please use these results of a growth curve experiment to answer the following questions Questions 1)...
please use these results of a growth curve experiment to
answer the following questions
Questions 1) Plot the OD600 readings and cell concentration for each time point on a scatterplot with a line connecting the points. Have the measured data (OD600 and cell concentration) on the y-axis against the time on the x-axis using Excel (or other computer based data/graphing program). It is helpful to set the y-axis on a log scale for the cell concentration measurements. 2) Can you...
this is a bacterial growth curve experiment . please explain rhe results Procedure: You will follow...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
Below is a table of results of E. coli grown in in nutrient broth (NB) and...
Below is a table of results of E. coli grown in in nutrient broth (NB) and minimal medium (MM) over 220 min. Time (min) A600 of NB MM 0 0.044 0.038 20 0.177 0.074 40 0.321 0.116 60 0.429 0.167 80 0.523 0.235 100 0.638 0.285 120 0.849 0.300 140 0.925 0.367 160 1.101 0.428 180 1.159 0.468 200 1.198 0.471 220 1.209 0.488 Using the above data, plot absorbance (log scale) versus time (linear), drawing both conditions on one...
The growth rate of Escherichia coli, a common bacterium found in the human intestine
Growth of Bacteria The growth rate of Escherichia coli, a common bacterium found in the human intestine, is proportional to its size. Under ideal laboratory conditions, when this bacterium is grown in a nutrient broth medium, the number of cells in a culture doubles approximately every 20 min. (a) If the initial population is 20, determine the function Q(t) that expresses the growth of the number of cells of this bacterium as a function of time t (in minutes). (b) How long would...
1:23 h Date Laboratory Report 45) Determination of a Bacterial Growth Curve and the Generation Time...
1:23 h Date Laboratory Report 45) Determination of a Bacterial Growth Curve and the Generation Time of a Culture 1. Collect your data on alsorance in the following table. Inbeton Time Absorbance 600 m 083 336 SIE .769 971 150 2. Estimate the generation time for this E. coli culture by the indirect method using your growth curve graph and extrapolations from the absorbances for doubling. Be sure to put UNITS on your answers. * From growth curve (indicate what...
E this experiment follows a modified procedure designed to demonstrate only the lag and log phase...
e this experiment follows a modified procedure designed to demonstrate only the lag and log phases by using an indirect method foe measuring growth. The indirect method is based on atisie at intervals during the incubation period. Such a procedure does not lend itself to a regular laboratory curve will be plotted usin appears cloudier as the bacteria increase in number by cell division. A tube of bacte- r can measure the n liquid culture, the medium amount of light...
1. (32 pts, total) Use the following information for the next 6 questions. The "rushing fireball"...
1. (32 pts, total) Use the following information for the next 6 questions. The "rushing fireball" is a motile prokaryote. It was originally isolated from a marine (ocean) environment. It does not fix carbon dioxide. It uses sugars and proteins as sources of energy, and it can oxidize elemental sulfur (S) or organic compounds for metabolism. It has a single cellular membrane surrounded by protein only. A graph comparing generations/doublings per day (vertical axis) with temperature (horizontal axis in degrees...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...
"Three organisms were innoculated on a culture media. Results showed Escherichia coli as red colonies, no...
"Three organisms were innoculated on a culture media. Results showed Escherichia coli as red colonies, no growth of Staphylococcus epidermidis, and colorless colonies of Salmonella enterica. The medium is:" a selective and differential b differential c selective d minimal e heterotrophic "A bacterium was incubated with aeration in a nutrient medium containing two carbon sources. After logarithmic growth, a lag phase was observed for 25 minutes. Then logarithmic growth at a slower rate was observed. Which substate was preferred by...
I need help answering the following microbiology questions. 1. The three tubes in the image to...
I need help answering the following microbiology
questions.
1. The three tubes in the image to the right contain glucose and the pH indicator phenol red. The middle and left tubes were inoculated with bacteria, and the right tube was left uninoculated as a control. Which of the tubes show evidence of heterolactic fermentation? a. Left tube Gas b. Middle tube c. Right tube d. None of the three tubes Yellow Red Red 2. Which of the three tubes in...
please use these results of a growth curve experiment to
answer the following questions
Questions 1) Plot the OD600 readings and cell concentration for each time point on a scatterplot with a line connecting the points. Have the measured data (OD600 and cell concentration) on the y-axis against the time on the x-axis using Excel (or other computer based data/graphing program). It is helpful to set the y-axis on a log scale for the cell concentration measurements. 2) Can you...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
1:23 h Date Laboratory Report 45) Determination of a Bacterial Growth Curve and the Generation Time of a Culture 1. Collect your data on alsorance in the following table. Inbeton Time Absorbance 600 m 083 336 SIE .769 971 150 2. Estimate the generation time for this E. coli culture by the indirect method using your growth curve graph and extrapolations from the absorbances for doubling. Be sure to put UNITS on your answers. * From growth curve (indicate what...
e this experiment follows a modified procedure designed to demonstrate only the lag and log phases by using an indirect method foe measuring growth. The indirect method is based on atisie at intervals during the incubation period. Such a procedure does not lend itself to a regular laboratory curve will be plotted usin appears cloudier as the bacteria increase in number by cell division. A tube of bacte- r can measure the n liquid culture, the medium amount of light...
1. (32 pts, total) Use the following information for the next 6 questions. The "rushing fireball" is a motile prokaryote. It was originally isolated from a marine (ocean) environment. It does not fix carbon dioxide. It uses sugars and proteins as sources of energy, and it can oxidize elemental sulfur (S) or organic compounds for metabolism. It has a single cellular membrane surrounded by protein only. A graph comparing generations/doublings per day (vertical axis) with temperature (horizontal axis in degrees...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...
I need help answering the following microbiology
questions.
1. The three tubes in the image to the right contain glucose and the pH indicator phenol red. The middle and left tubes were inoculated with bacteria, and the right tube was left uninoculated as a control. Which of the tubes show evidence of heterolactic fermentation? a. Left tube Gas b. Middle tube c. Right tube d. None of the three tubes Yellow Red Red 2. Which of the three tubes in...
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