Question

1. (32 pts, total) Use the following information for the next 6 questions. The "rushing fireball" is a motile prokaryote. It was originally isolated from a marine (ocean) environment. It does not fix carbon dioxide. It uses sugars and proteins as sources of energy, and it can oxidize elemental sulfur (S) or organic compounds for metabolism. It has a single cellular membrane surrounded by protein only. A graph comparing generations/doublings per day (vertical axis) with temperature (horizontal axis in degrees Celsius) is shown in Fig. 1. An image of Microbe T obtained under vacuum is shown in Fig. 2. The white bar in the lower right corner of the images presented in Fig. 2. A and B indicate 5 um and 2 um, respectively. J. Bacteriol. 2006 October 188(19): 6915-6923. doi: 10.1128/JB.00527-06) Fig. 2 Fig. 1 Generations per day glish (United States) D Focus op ^ ' 93

Answer 1

3)d) late log phase (because from 8am to 8pm, microbe will go through about 20generation calculated by diving 1440(time period)/36(doubling time). In these 20 generation, it will produce about 10^7cells/ml (no of cells=No of intial cells*2^(no of generation) as we know stationary phase generally come when cell volume reaches 10^9/ml, so it has to be late log phase.

4)a) salmonella typhimurium

5). c) archea

6) e) both c)and d) above are correct ( as mentioned in above paragraph that it utilises both elemental sulphur(inorganic) and organic compound for metabolism)

1) option doesnt seems available

2) d)1.6 (its correct)