Question

A solution of Red Dye 40 is analysed via UV-Vis spectroscopy, and is found to have an absorbance of 0.192. If the absorp- tion coefficient (£) for Red Dye 40 is 11 502 M2 cm- and the length of the cuvette used is 1.00 cm, what is the concentra- tion of Red Dye 40 in the solution? (You will need to read the pre-lab material to solve this problem.)

Answer 1

The given problem will be solved by using the Beer lambert's formula - Az Elu where Az absurbance = 0.192 E= abssuption coefficient for red bye to = 11502mich c= concentration of red sye to=? l= length of cuvette = 1.00cm Plugging the values in equ (i) we get -0.192 = 11502m'cut x CX1:00 ch cs 0.192 = 1.67 x 10 m 71502 m'x100 Hence; concentration of the Red dye is 1.67x10 m 1.