Define the terms mobile phase, adsorbent, and solid support in terms of a general chromatography experiment.
Define the terms mobile phase, adsorbent, and solid support in terms of a general chromatography experiment.
Homework Answers
Chromatography is a method of seperation of a chemical mixture.
Adsorbent: Adsorbent is a substance with high surface area and porous in nature and have ability to adsorb substance onto its surface.
Mobile phase: In chromatography the mixture is seperated by distributing components between statonary and mobile phases. The mobile phase carries a component of mixture over the stationary phase
Solid support:The support on which the stationary phase is placed is solid support. In some cases the solid support itself is stationary phase
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Define the terms mobile phase, adsorbent, and solid support in
terms of a general chromatography experiment.
chromatography 7 200 mm quee Rf 2. Define the stationary phase, Rfval and mobile phase in...
chromatography 7 200 mm quee Rf 2. Define the stationary phase, Rfval and mobile phase in Anomatography
3. There are two major components in the chromatography experiment: the solvent system (mobile phase) and...
3. There are two major components in the chromatography experiment: the solvent system (mobile phase) and the paper (stationary phase). Which of these major components is more polar phase and which is less polar phase? Explain using Lewis structures. (please PLEASE use the lewis structures, thats the part of the question I don't have yet!) 4. What parts of the molecules in the stationary phase will interact with the molecules that are being separated? Please answer this question using the...
Problem 1 Thin layer Chromatography consist of three parts: The analyte, the stationary phase and mobile...
Problem 1 Thin layer Chromatography consist of three parts: The analyte, the stationary phase and mobile phase. Match each of these terms to what is was in out experiment. Stationary Phase ____ a) The solvent Mobile Phase ____ b) Silica Analyte ____ c) One of the analgesiscs Problem 2 Complete the sentences Analytes on a TLC should have a(n) _________ Rf in a less polar solvent. Analytes on a TLC should have a(n) _________ Rf in a more polar solvent....
Please draw a thin chromatography plate with the following terms labelled 1. Origin 2. Stationary Phase...
Please draw a thin chromatography plate with the following
terms labelled
1. Origin 2. Stationary Phase 3. Mobile Phase 4. Solvent Front 5. Component "spot" 6. Rf value 7. Development 8. Visualization of spots Define questions 9 and 10 9. What is meant by differential partitioning between stationary and mobile phase? 10. What is the recommended procedure for cleaning a TLC spotting capillary?
Select the correct characteristics describing normal-phase and reversed-phase chromatography. Normal-phase chromatography Reversed-phase chromatography (a) Polarity of...
Select the correct characteristics describing normal-phase and reversed-phase chromatography. Normal-phase chromatography Reversed-phase chromatography (a) Polarity of phases: Stationary phase is polar Stationary phase is non polar b) Eluent strength of solvent. Increases as solvent becomes more polar. Increases as solvent becomes less polar c) Nature of solutes. Polar Non-polar d) Nature of solute interaction. More soluble in mobile phase as the polarity of the mobile phase increases. More soluble in mobile phase as the polarity of the mobile phase decreases....
aestion 7 8 poir Find the chromatography method for following description. Solute equilibrates between the mobile...
aestion 7 8 poir Find the chromatography method for following description. Solute equilibrates between the mobile phase and the surface of the solid stationary phase. ion-exchange chromatography A. partition chromatography adsorption chromatography C. o size exclusion chromatography
5. Liquid Chromatography. b. For a reverse-phase HPLC separation with a mobile phase pH = 3.00,...
5. Liquid Chromatography. b. For a reverse-phase HPLC separation with a mobile phase pH = 3.00, circle which of the following compounds would elute first and briefly describe why: Propanoic acid (CH3CH2COOH, PK. = 4.87) or Propylamine (CH2CH2CH2NH2, pks = 3.43). b. If one uses gradient elution with normal phase chromatography, would it be better to go from a less polar to a more polar solvent or a more polar to a less polar solvent? Why?
In liquid chromatography, the solvent (mobile phase) composition often changes with time during each run. What...
In liquid chromatography, the solvent (mobile phase) composition often changes with time during each run. What parameter usually changes with run time in gas chromatography and why?
Ion-interaction chromatography can be used to separate out ions.(i) Discuss this mode of chromatography in terms...
Ion-interaction chromatography can be used to separate out ions.(i) Discuss this mode of chromatography in terms of retention mechanisms, ion-pairing reagents, column type and mobile phase pH requirements.(ii) Describe how this method differs from ion-suppression chromatography.
Define stationary phase. O Area where the points don't move on the chromatography paper O A...
Define stationary phase. O Area where the points don't move on the chromatography paper O A solvent or mixture of solvents that flows over the moving phase. A solid phase of the system usually composed of a small piece of clay. porcelain, cellulose or other material 7. value: Required information 10.00 points The separation of a mixture occurs using chromatography because each substance in the mixture will be adsorbed to a different degree on the surface of the stationary phase....
chromatography 7 200 mm quee Rf 2. Define the stationary phase, Rfval and mobile phase in Anomatography
Please draw a thin chromatography plate with the following
terms labelled
1. Origin 2. Stationary Phase 3. Mobile Phase 4. Solvent Front 5. Component "spot" 6. Rf value 7. Development 8. Visualization of spots Define questions 9 and 10 9. What is meant by differential partitioning between stationary and mobile phase? 10. What is the recommended procedure for cleaning a TLC spotting capillary?
Select the correct characteristics describing normal-phase and reversed-phase chromatography. Normal-phase chromatography Reversed-phase chromatography (a) Polarity of phases: Stationary phase is polar Stationary phase is non polar b) Eluent strength of solvent. Increases as solvent becomes more polar. Increases as solvent becomes less polar c) Nature of solutes. Polar Non-polar d) Nature of solute interaction. More soluble in mobile phase as the polarity of the mobile phase increases. More soluble in mobile phase as the polarity of the mobile phase decreases....
aestion 7 8 poir Find the chromatography method for following description. Solute equilibrates between the mobile phase and the surface of the solid stationary phase. ion-exchange chromatography A. partition chromatography adsorption chromatography C. o size exclusion chromatography
5. Liquid Chromatography. b. For a reverse-phase HPLC separation with a mobile phase pH = 3.00, circle which of the following compounds would elute first and briefly describe why: Propanoic acid (CH3CH2COOH, PK. = 4.87) or Propylamine (CH2CH2CH2NH2, pks = 3.43). b. If one uses gradient elution with normal phase chromatography, would it be better to go from a less polar to a more polar solvent or a more polar to a less polar solvent? Why?
Define stationary phase. O Area where the points don't move on the chromatography paper O A solvent or mixture of solvents that flows over the moving phase. A solid phase of the system usually composed of a small piece of clay. porcelain, cellulose or other material 7. value: Required information 10.00 points The separation of a mixture occurs using chromatography because each substance in the mixture will be adsorbed to a different degree on the surface of the stationary phase....
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