Question

4. Every enzyme has an optimal pH at which they function, which can vary widely for many different proteins. Some enzymes function best at low pH while others function best at high pH. Provide an explanation as to why this is and include in your answer a discussion of protonation states (specifically within the active sites). (2pts)

Answer 1

Page No. Date Ansulen- some engumes function best et low pH while otheus function best at High pH this is due to :- 0 pH has two Sepanote effects of enzymes, that may ou may not be easy to disteit dange pH changes affect protein &tability t. digand binding and proteins can be very Specific ligands) can add stability to the complex s and such is the hasy of the thermal Shift assay. #bH dependance is usually due to the side groups of the amino auds · A change in PbH changes the protonation patteun and con in extreme cases, lles wts in Protein denaturation, Therefore suspect that your protein stabilizes the enzyme Structure, this keeping it active at fub optimal pH values. change in pH Change the so elected point of Yen24 mes. Tans is the Meason eveny enzymes has y enzymes has - an optimal pH.