Question

1. How would you prepare 250mL’s of a 12% sodium citrate solution?

2. Your stock DNA is at 850μg/ml. You want to make 100μl of a 50ng/μl stock, using TE as your solvent. How would you do this?

3. You have a 10mg/ml sucrose solution. How would you prepare 15ml of a 10 μg/ml sucrose solution?

Not good at the math part of these questions, please help!

Answer 1

10100ml Solution Require 129. Na-Citrate 1:250 mL " Requireyiz , 2501-30. Na-Cibrate They, 309. Sceliem citite required to prepare 250mL 12% - Sodium Citrate Solutions

1 2.850mg/ml = 0.85 Mg /uL=850ng.ful . Required volume of song. ML Stock DNA = 100.450 - 5-88 ] Olt

Thus, 5.88$\mu$L of 850$\mu$g/mL Stock Solution is to be mixed with (100-5.88)= 94.12$\mu$L of TE Solvent.

3. 10mg/mL = (0.103) ug/mL - Pequired volume olomy./mL Sueroa Shitesk = 1500 = 0-075 m2 4 Tspill

Thus, 15$\mu$L of the 10 mg/mL Sucrose Solution is to be diluted with (15000-15)= 14985$\mu$L of Water.