Question

Question 33 of 33 Using the standard reduction potential (E° = -0.105 V) and the biochemical formal potential (Eº'= -0.320 V) for NAD+ reduction reaction, determine the pH of the biochemical environment. 1 2 3 C 789 +/- +/- 10 x 100

Answer 1

Here to solve this question we must have the basic knowledge of electrochemistry. Firstly the reduction reaction of NAD+ is written and then all the given values are substituted in the Nernst equation appropriately and after substituting these values we are able to calculate the pH of the biochemical environment

Here the standard seduction potential E° 2-00105 v the biochemical foomal potential Ĉ--003201 dt pas le now to find the pH we need to use neunst een jieg £ £'-9'3031 og Creactants] 7.e9 € = &- 9:303 RTlog Lipasoducts] no here the son involved is 20 tHt+ Naot NADH thus substituting the above values in the esh £09 Eº - 2.303x8-314x298 log Ena DHL Q x 96506 [NAD+] [sit as the temp is not mentioned in question thus assuming the tempo atuwe to 290k & Conc. of Nada ] & [NADH] = 1M at biochemic going back to equation ennisonn - 00320= -0.10500059) Tog L HT

we know that 109 1 1 7 = -log CH l] =p4 so -003202 -0.105 -0.02gssph the egn becomes on solving the equation me guation r - TPH 27.27) at bait at are piti sovint soko glais je ne CaoutIts do solta asukor svado ant scritstitadudo avat See les EOEC - cotouer ontol angolo - des station compte leigence 2010 - S Sv 0 -