Question

alpha-Ketoglutarate plays a central role in the biosynthesis of several amino acids. Write a sequence of enzymatic reactions that could results in the NET synthesis of alpha-ketoglutarate from pyruvate. You may use other cofactors as required (e.g. NADH, ATP). There is no need to show structures, but indicate the name of each intermediate, the name of the enzyme, and balance each reaction for cosubstrates and cofactors. Each of the carbons in your alpha-ketoglutarate product must have originated from pyruvate.

Answer 1

Step. Synthesis of cupha ketoglutarete yon pyruvale Pyruvato) suunapt foz twadht (acetyl COA G-S-COA coo Pyruvate C-G-S-COA c=0 dehydrogenars ch enzyme COAT-SH © (acetyl com) C-9-5-10A (citrate) cool citrate synthetare enzyme си. HO-C-(oo (H2 cool -420 (citrate) coo- - aconitase (cis aconitate) Соо- CH2 enzyme to-c-coo (12 (-100- си.- си cool cool

(cis-aconitate) coot - & 4420 , (iso citrate) Coo aconitate си, enzyme c-600 си, Hc-cool 10-C-H coor CH coo- ② (isocitrate) - NADT NADH,HT (02 iso citrate dehydrogenase enzyme (x-ketoglutar tate) 000 42 CH2 4c-cool HOC-n 620 coo coo There are only a NADH formed in reaction (INADH equivalent of 2.5 ATP)

Enzyme Substrate Oxaloaletate t Acetyl COAT H2O product citrate icon citrate Synthase SH citrate cis Aconitase +H2O cis Aconitate +H2O isocitrate Aconiture iso citrate TNADT o xalosur cinate INADH THT T iso citrate dehydrogenase oxalasuccinate & -ketoglutarate tco2