2. Calculate the amount of cholesterol standard needed to prepare each of the samples for the...

Question

Question

2. Calculate the amount of cholesterol standard needed to prepare each of the samples for the cholesterol assay. The std solu

Answer 1

Answer #1

Answer :

Total ug of cholesterol is calculated as :

Concentration of Std. Solution (ug/uL ) * amount of Std . Solution (uL)

Amount of Std. Solution (uL ) = total ug of cholesterol (ug) / concentration of Std. Solution (ug/uL)

Concentration of Std soluSolu is fixed = 5ug/uL

In each step we have to calculate amount of Std. Solution required (uL)

Then add water to make the overall Solution volume to be 20uL

(1) : To obtaine 100 ug of cholesterol

Amount of Std. Solution required (uL ) = total ug of cholesterol / concentration of Std. Solution (ug/uL) = 100ug / (5ug/uL) = 20 uL

So no need to add water into this solution.

Total ug of cholesterol	amount of Std. Solution required (uL)	Amount of water required (uL)
0	=> 0 / 5 = 0	20
20	=> 20 / 5 = 4	16
40	=> 40 / 5 = 8	12
60	=> 60 /5 = 12	8
80	=> 80 / 5 = 16	4
100	=> 100 / 5 = 20	0

Answer 2

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