How to prepare these dilutions? 1. With access to 100 ppm riboflavin solution (50 mg riboflavin...
How to prepare these dilutions?
1. With access to 100 ppm riboflavin solution (50 mg riboflavin and 2.5 mL glacial acetic acid in 500 mL of distilled water), Prepare 25.00 mL of the riboflavin solution at 20 ppm in the 1:1 mixture of distilled water : 10% phosphate buffer. (The previous step was to make a 1:1 mixture of distilled water : 10% phosphate buffer of 30 mL)
2. Prepare 25.00 mL of a 100 ppb stock solution from the 100 ppm stock solution, diluting with only distilled water. (A two-step dilution is necessary)
Homework Answers
1. From 100pm to 20ppm riboflavin solution , take 5 ml of 100ppm solution and mix it with 20ml distilled water to prepare 25ml of 20ppm solution
2. 1ppm= 1000ppb
This we have to prepare 0.1ppm , 25ml from a 100ppm solution
Take 0.025ml of 100ppm stock and add it to 24.975ml distilled water
Add Answer to:
How to prepare these dilutions?
1. With access to 100 ppm riboflavin solution (50 mg riboflavin...
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in...
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a...
how do I prepare the 100mg/L of asprin acetaminophen and caffeine solution and dilute that solution...
how do I prepare the 100mg/L of asprin acetaminophen and
caffeine solution
and dilute that solution with 1a% acetic acid to creat 50, 10
and 5mg/L solutions
Preparation of Standards: Using a 100ML volumetric flask and 1 % acetic acid solution (prepared in hood from glacial acetic acid using a 1000mL beaker and 10mL graduated cylinder), prepare and label a stock solution of 100 mg/L each of aspirin, acetaminophen, and caffeine combined together in a single volumetric flask. Using appropriate...
QUESTION5 Starting with a stock solution containing 60 mg/dL of glucose, and using dH2O as your...
QUESTION5 Starting with a stock solution containing 60 mg/dL of glucose, and using dH2O as your diluting solution, you need to make 6 mL of each of the following glucose solutions: 60 mg/dL, 30 mg/dL, 15 mg/dL, 7.5 mg/dL, 3.75 mg/dL, and 1.875 mg/dL.. What is the volume of arrow A? 3 O a 12 mL Ob. 24 mL C(V1-V2) x df ⓔd.6mL QUESTION6 Starting with a stock solution containing 60 mg/dL of glucose, and using dH20 as your diluting...
dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
1) Figure below show a 36 uL of stock solution containing 800 ppm of CuSO4 was...
1) Figure below show a 36 uL of stock solution containing 800 ppm of CuSO4 was transferred into T1 tube contained with 324 uL of wash buffer. Next, serial dilution was performed from T1 to T5. (CuSO4 molar mass: 159.61 g/mol) 36 ul 120 ML 120 ML 120 L 120 L 800 ppm Cuso T1 Stock solution T2 T3 T4 TS B1 Dilution Factor Final Concentration NA 800 ppm Tube Volume Diluent Total Final Transferred Volume Volume Volume (PL) (wash...
Tablet Information(75 Tablets -each tablet contains 5.1mg riboflavin ), concentration of riboflavin in unknown powder solution:...
Tablet Information(75 Tablets
-each tablet contains 5.1mg riboflavin ), concentration of
riboflavin in unknown powder solution: 1.01±0.04ppm. Find the
Report the mass of riboflavin in your tablet.
3. Preparation of standard addition solutions. Prepare six 25 mL volumetric flasks as follows: Volume of tablet solution (mL) 2.00 2.00 2.00 2.00 2.00 2.00 Volume of standard riboflavin solution (mL) 0 1.00 2.50 7.50 12.50 20.00 Top each flask to the mark Measure the volume of standard riboflavin solution using a burette....
Start with a 1230.0 ppm Stock Zn solution. Prepare control 1 by diluting 2.00 mL of Stock Zn solution in a 200.0 mL...
Start with a 1230.0 ppm Stock Zn solution. Prepare control 1 by diluting 2.00 mL of Stock Zn solution in a 200.0 mL volumetric flask and dilute to the mark. Prepare control 2 by diluting 2.00 mL of control 1 into a 100.0 mL volumetric flask. What is the Zn concentration (ppm Zn) in control 2? Report your answer to 4 decimal place.
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
Answer 3) The concentration of phosphorous in a stock solution is 10.0 ppm. After the dilution...
Answer
3) The concentration of phosphorous in a stock solution is 10.0 ppm. After the dilution by DI water, three new solutions are prepared as shown in the following table. Find the concentration of phosphorous in these solutions. Show all calculation processes to receive the credit. (6 pts) Phosphorous Concentration After dilution (ppm) Solution # Stock Solution (mL) Total Volume After dilution (ml.) 25.00 2.50 1 50.00 2 8.00 250.0 3 50.00
How would you prepare a 1/100 dilution (of a concentrated stock solution) in a final vol...
How would you prepare a 1/100 dilution (of a concentrated stock solution) in a final vol of 100 ml? 2.5 L? 87 ml? (ie how much diluent and how much of your concentrated stock)?
1. How would you prepare 125 ml of a 10% (v/v) solution of acetic acid in H2O? 2. How would you prepare 60 ml of a 5% (W/V) NaCl solution in H20? 3. How would you prepare 3 ml of 82% solution A and 18% solution B? 4. 5 ml of a 2.0 mg/ml protein solution contains mg protein. a. If 1.0 ug/ml of pigment X shows an absorbance of 0.059 at 370nm, then what is the concentration of a...
how do I prepare the 100mg/L of asprin acetaminophen and
caffeine solution
and dilute that solution with 1a% acetic acid to creat 50, 10
and 5mg/L solutions
Preparation of Standards: Using a 100ML volumetric flask and 1 % acetic acid solution (prepared in hood from glacial acetic acid using a 1000mL beaker and 10mL graduated cylinder), prepare and label a stock solution of 100 mg/L each of aspirin, acetaminophen, and caffeine combined together in a single volumetric flask. Using appropriate...
QUESTION5 Starting with a stock solution containing 60 mg/dL of glucose, and using dH2O as your diluting solution, you need to make 6 mL of each of the following glucose solutions: 60 mg/dL, 30 mg/dL, 15 mg/dL, 7.5 mg/dL, 3.75 mg/dL, and 1.875 mg/dL.. What is the volume of arrow A? 3 O a 12 mL Ob. 24 mL C(V1-V2) x df ⓔd.6mL QUESTION6 Starting with a stock solution containing 60 mg/dL of glucose, and using dH20 as your diluting...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
1) Figure below show a 36 uL of stock solution containing 800 ppm of CuSO4 was transferred into T1 tube contained with 324 uL of wash buffer. Next, serial dilution was performed from T1 to T5. (CuSO4 molar mass: 159.61 g/mol) 36 ul 120 ML 120 ML 120 L 120 L 800 ppm Cuso T1 Stock solution T2 T3 T4 TS B1 Dilution Factor Final Concentration NA 800 ppm Tube Volume Diluent Total Final Transferred Volume Volume Volume (PL) (wash...
Tablet Information(75 Tablets
-each tablet contains 5.1mg riboflavin ), concentration of
riboflavin in unknown powder solution: 1.01±0.04ppm. Find the
Report the mass of riboflavin in your tablet.
3. Preparation of standard addition solutions. Prepare six 25 mL volumetric flasks as follows: Volume of tablet solution (mL) 2.00 2.00 2.00 2.00 2.00 2.00 Volume of standard riboflavin solution (mL) 0 1.00 2.50 7.50 12.50 20.00 Top each flask to the mark Measure the volume of standard riboflavin solution using a burette....
Start with a 1230.0 ppm Stock Zn solution. Prepare control 1 by diluting 2.00 mL of Stock Zn solution in a 200.0 mL volumetric flask and dilute to the mark. Prepare control 2 by diluting 2.00 mL of control 1 into a 100.0 mL volumetric flask. What is the Zn concentration (ppm Zn) in control 2? Report your answer to 4 decimal place.
Answer
3) The concentration of phosphorous in a stock solution is 10.0 ppm. After the dilution by DI water, three new solutions are prepared as shown in the following table. Find the concentration of phosphorous in these solutions. Show all calculation processes to receive the credit. (6 pts) Phosphorous Concentration After dilution (ppm) Solution # Stock Solution (mL) Total Volume After dilution (ml.) 25.00 2.50 1 50.00 2 8.00 250.0 3 50.00
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