Question

How would you prepare a 1/100 dilution (of a concentrated stock solution) in a final vol of 100 ml? 2.5 L? 87 ml? (ie how much diluent and how much of your concentrated stock)?

Answer 1

To create a 1/100 dilution in

100 ml: We need to add 1 ml stock solution to 99 ml water (dilutent).

2.5 L: Since for every 100 ml we need 1 ml stock solution, for 2500 ml we will need 25 ml stock solution. That means that 25 ml stock solution will be added to 2475 ml water.

87 ml: Similarly, 0.87 ml or 870 $\mu$l stock solution if added to 86.13 ml water, we would get a solution diluted by 1/100 diluted factor.