Homework Answers
The labelled carbon C14 appeared in first carbon of
citrate .the pathway outlined below 
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42. (5 pts) Ribose-5-Phosphate, labeled at the 4th carbon with 'C, was added to rat liver...
Excess dietary fat can be converted into cholesterol in the liver. When palmitate labeled with^14C at...
Excess dietary fat can be converted into cholesterol in the
liver. When palmitate labeled with^14C at every odd-numbered carbon
is added to liver homogenate, where does the radiolabel appear in
mevalonate?
which structure is correct and why?
are the radiolabelled carbons at C 1 3 5 or at C 2 3 5?
The following structure indicates position of labeled "C at carbon atom. Palmitate will be broken down into acetyl CoA, which will then be incorporated into mevalonate. CoASH Mevalonate...
In a "pulse chase" experiment under anaerobic conditions, 14C glucose is added to an ethanol-producing yeast...
In a "pulse chase" experiment under anaerobic conditions, 14C glucose is added to an ethanol-producing yeast cell extract just long enough that each intermediate in the ethanol fermentation is labeled with a 14C (pulse). The 14C label is then diluted so that no additional radioactivity is incorporated into the intermediates (chase). 1. What position in ethanol will be labeled with 14C if 14C-glucose labeled at position C-1 is used? 2. Where should the 14C label sit in glucose for it...
Starting from Alpha Ketoglutarate please make Ribose-5-Phosphate by listing enzymes in the step and products. 10...
Starting from Alpha Ketoglutarate please make
Ribose-5-Phosphate by listing enzymes in the step and products.
10 pts Question 47 Starting from Alpha Ketoglutarate make Ribose-5-Phosphate Instructions for entering answers: . Any major substrate you wish to use, you must have been provided or your must shown it being made. • Provided materials do not necessarily need to be used in the pathway. . Do not use capitals or punctuation (e.g. acetyl coa is correct Acetyl-CoA will be marked wrong) Do...
QUESTION 2 (A) The pentose phosphate pathway is comprised of two phases. Why are both phases...
QUESTION 2 (A) The pentose phosphate pathway is comprised of two phases. Why are both phases necessary for the survival of the cell? (2pts) (B) In the non-oxidative phase of the pentose phosphate pathway ribose 5-phosphate is converted to fructose 6-phosphate. Why does this pathway require so many enzymatic steps? What benefit does each step provide? (4 pts) (C) Many cancer drugs are effective because they reduce cell proliferation by reducing the amount of base material needed for cell division...
There are 3 parts to this question (AC) 5. Concerning the pentose phosphate pathway. A In...
There are 3 parts to this question (AC) 5. Concerning the pentose phosphate pathway. A In the nonoxidative phase of the pentose phosphate pathway, from where does the xylulose-5-phosphate come? B. At the end of the nonoxidative portion of the pentose phosphate pathway the result is 2 molecules of fructose-6-phosphate and 1 molecule of glyceraldehyde-3-phosphate Toget this, how many times did the oxidative phase have to run lie, how many glucose-6-phosphate molecules were used? C. How many total NADPH will...
5. Glycolysis a. The hydroxyl group on the sixth carbon on a glucose molecule is radiolabeled...
5. Glycolysis a. The hydroxyl group on the sixth carbon on a glucose molecule is radiolabeled using O. Please draw out the pathway showing where this radiolabel is in each step of the pathway, b. In glycolysis, ATP molecules are produced. Please write out the reactions that utilize ATP as well as the ones that create ATP, and explain how many molecules of ATP are made in glycolysis. c. A reaction is set up with 3-phosphoglycerate, where the phosphate atom...
NADP+ NADPHH HC-OH HO-CH HC-OH нс-ОН CH2OPO;? 6PGDH 'Coz CH OH c=0 HC-OH HC-OH CHOPO3 In...
NADP+ NADPHH HC-OH HO-CH HC-OH нс-ОН CH2OPO;? 6PGDH 'Coz CH OH c=0 HC-OH HC-OH CHOPO3 In pentose phosphate pathway, 6-phosphogluconate undergoes oxidation and decarboxylation by 6- phosphogluconate dehydrogenase (6PGDH) to form D-ribulose 5-phosphate. The reaction generates NADPH, as shown in the scheme. (1) Fill the blanks. During the 6PGDH-catalyzed reaction, the A group of carbon (B) of 6-phosphogluconate is oxidized to the group in ribulose 5-phosphate. A: (name of the functional group). B: (carbon numbering) C: (name of the functional...
5) The rate of reaction of hemoglobin (Hb) with carbon monoxide was studies at 20°C. The...
5) The rate of reaction of hemoglobin (Hb) with carbon monoxide was studies at 20°C. The following data was collected, with all concentrations in units of 10-6 mol/L. a) Calculate the rate equation, including the value of the rate constant, from the data shown. (20 pts) [Hb). 2.21 4.42 4.42 (CO). 1.00 1.00 3.00 Initial Rate 0.619 1.24 3.71 b) What would be the rate for an experiment where the initial (HB) = 3.36 x 106 and (CO). = 2.40...
please help with these chemistry problems 2. (5 points) Why does this reaction occur via a...
please help with these chemistry problems
2. (5 points) Why does this reaction occur via a Si1 pathway and not via a Si2 pathway? 3. (5 points) In general are SN1 reactions stereospecific? Why or why not? 2 5.(5 points) Why do carbon substituents stabilize a carbocation and destabilize a carbanion? 6. (5 points) The mass spec of your product has a base peak at 77 draw what this fragment looks like. What would explain the peak at 79. (you...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter your results in the table below. Organism Color Result (+ or -) Interpretation Control QUESTIONS 1 Consider the uninoculated tube. a. Is it a positive or a negative control? b. What information is provided by the uninoculated control? Rus. ay Manry bacteria that are able to metabolize citrate (as seen in the citric acid cycle) produce negative e this test. Why? Be specific. ative...
Excess dietary fat can be converted into cholesterol in the
liver. When palmitate labeled with^14C at every odd-numbered carbon
is added to liver homogenate, where does the radiolabel appear in
mevalonate?
which structure is correct and why?
are the radiolabelled carbons at C 1 3 5 or at C 2 3 5?
The following structure indicates position of labeled "C at carbon atom. Palmitate will be broken down into acetyl CoA, which will then be incorporated into mevalonate. CoASH Mevalonate...
Starting from Alpha Ketoglutarate please make
Ribose-5-Phosphate by listing enzymes in the step and products.
10 pts Question 47 Starting from Alpha Ketoglutarate make Ribose-5-Phosphate Instructions for entering answers: . Any major substrate you wish to use, you must have been provided or your must shown it being made. • Provided materials do not necessarily need to be used in the pathway. . Do not use capitals or punctuation (e.g. acetyl coa is correct Acetyl-CoA will be marked wrong) Do...
QUESTION 2 (A) The pentose phosphate pathway is comprised of two phases. Why are both phases necessary for the survival of the cell? (2pts) (B) In the non-oxidative phase of the pentose phosphate pathway ribose 5-phosphate is converted to fructose 6-phosphate. Why does this pathway require so many enzymatic steps? What benefit does each step provide? (4 pts) (C) Many cancer drugs are effective because they reduce cell proliferation by reducing the amount of base material needed for cell division...
There are 3 parts to this question (AC) 5. Concerning the pentose phosphate pathway. A In the nonoxidative phase of the pentose phosphate pathway, from where does the xylulose-5-phosphate come? B. At the end of the nonoxidative portion of the pentose phosphate pathway the result is 2 molecules of fructose-6-phosphate and 1 molecule of glyceraldehyde-3-phosphate Toget this, how many times did the oxidative phase have to run lie, how many glucose-6-phosphate molecules were used? C. How many total NADPH will...
5. Glycolysis a. The hydroxyl group on the sixth carbon on a glucose molecule is radiolabeled using O. Please draw out the pathway showing where this radiolabel is in each step of the pathway, b. In glycolysis, ATP molecules are produced. Please write out the reactions that utilize ATP as well as the ones that create ATP, and explain how many molecules of ATP are made in glycolysis. c. A reaction is set up with 3-phosphoglycerate, where the phosphate atom...
NADP+ NADPHH HC-OH HO-CH HC-OH нс-ОН CH2OPO;? 6PGDH 'Coz CH OH c=0 HC-OH HC-OH CHOPO3 In pentose phosphate pathway, 6-phosphogluconate undergoes oxidation and decarboxylation by 6- phosphogluconate dehydrogenase (6PGDH) to form D-ribulose 5-phosphate. The reaction generates NADPH, as shown in the scheme. (1) Fill the blanks. During the 6PGDH-catalyzed reaction, the A group of carbon (B) of 6-phosphogluconate is oxidized to the group in ribulose 5-phosphate. A: (name of the functional group). B: (carbon numbering) C: (name of the functional...
5) The rate of reaction of hemoglobin (Hb) with carbon monoxide was studies at 20°C. The following data was collected, with all concentrations in units of 10-6 mol/L. a) Calculate the rate equation, including the value of the rate constant, from the data shown. (20 pts) [Hb). 2.21 4.42 4.42 (CO). 1.00 1.00 3.00 Initial Rate 0.619 1.24 3.71 b) What would be the rate for an experiment where the initial (HB) = 3.36 x 106 and (CO). = 2.40...
please help with these chemistry problems
2. (5 points) Why does this reaction occur via a Si1 pathway and not via a Si2 pathway? 3. (5 points) In general are SN1 reactions stereospecific? Why or why not? 2 5.(5 points) Why do carbon substituents stabilize a carbocation and destabilize a carbanion? 6. (5 points) The mass spec of your product has a base peak at 77 draw what this fragment looks like. What would explain the peak at 79. (you...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter your results in the table below. Organism Color Result (+ or -) Interpretation Control QUESTIONS 1 Consider the uninoculated tube. a. Is it a positive or a negative control? b. What information is provided by the uninoculated control? Rus. ay Manry bacteria that are able to metabolize citrate (as seen in the citric acid cycle) produce negative e this test. Why? Be specific. ative...
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