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The lac operon is an example of negative regulation. Based on what you know about the...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
Consider what you know about the lac operon, and predict whether the repressor and the activator...
Consider what you know about the lac operon, and predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels of transcription. and the activator is b. When lactose and glucose are...
61. The lac operon a. is an example of negative control. b. is affected by catabolite...
61. The lac operon a. is an example of negative control. b. is affected by catabolite repression c. produces lactose. d. is an example of a regulon. e. a and b Bonus questions: nepxample of its use. (0.5 pts) Describe biotechnology and list only one example of its use. es that (0.5 pts) Why are bacteria so diverse and list one example of such diversity?
Imagine you are carrying out research on the lac operon. You isolate six mutations in the...
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
Most of what we know about the lac operon in E. coli has come from the...
Most of what we know about the lac operon in E. coli has come from the genetic analysis of various mutants. Below is a list of mutants for regions of the operon. A + superscript indicates no mutation and normal function of that region, - indicates a knock out and no function of that region, c indicates the mutation resulted in constitutive action of that region. The effect of the mutation is determined by expression of the lacZ gene as...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high glucose d) Low lactose, low glucose e) None of the above would have any lac operon expression 2. (2 points) Which of the following is true concerning molecular genetics? a) tRNA carries amino acids into the nucleus in eukaryotic cells b) DNA polymerase moves towards the...
The following is a diagram of the LCT (lactase gene) gene regulation system in humans. When...
The following is a diagram of the LCT (lactase gene) gene
regulation system in humans. When the LCT gene is transcriptionally
active, the cell produces the enzyme lactase, which is
needed to digest the lactose in many dairy products. Based on what
you know about enhancers and promoters from your reading, predict
how the following scenarios might influence LCT gene expression and
thus lactase production in cells:
The enhancer sequence is inverted, but remains intact so that
Oct1 still binds...
Which of the following statements about Huntington's is true? You can retry this question, the average...
Which of the following statements about Huntington's is true? You can retry this question, the average of your two answers is recorded. You should see feedback if you get a question wrong. O a. The mutated allele(s) has/have lost the function of keeping brain cells healthy, and is/are therefor a dominant disease O b. Huntington is a very rare and therfor recessive. c. Huntington strikes people after reproductive age and is therefor dominant. d. The mutated allele(s) has/have gained the...
Page < 2 > of 2 D. The trp operon would be expressed at high levels,...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
Consider what you know about the lac operon, and predict whether the repressor and the activator is active (able to bind to DNA) or inactive (unable to bind to DNA), and the level of transcription in the following scenarios. Options for transcription levels include high, medium, leaky and off. a. When lactose is present but glucose is not, the repressor is and the activator is leading to levels of transcription. and the activator is b. When lactose and glucose are...
61. The lac operon a. is an example of negative control. b. is affected by catabolite repression c. produces lactose. d. is an example of a regulon. e. a and b Bonus questions: nepxample of its use. (0.5 pts) Describe biotechnology and list only one example of its use. es that (0.5 pts) Why are bacteria so diverse and list one example of such diversity?
Imagine you are carrying out research on the lac
operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
Quiz 10 1. (2 points) Which of the following conditions would lead to the highest levels of lac operon expression? a) High lactose, high glucose b) High lactose, low glucose c) Low lactose, high glucose d) Low lactose, low glucose e) None of the above would have any lac operon expression 2. (2 points) Which of the following is true concerning molecular genetics? a) tRNA carries amino acids into the nucleus in eukaryotic cells b) DNA polymerase moves towards the...
The following is a diagram of the LCT (lactase gene) gene
regulation system in humans. When the LCT gene is transcriptionally
active, the cell produces the enzyme lactase, which is
needed to digest the lactose in many dairy products. Based on what
you know about enhancers and promoters from your reading, predict
how the following scenarios might influence LCT gene expression and
thus lactase production in cells:
The enhancer sequence is inverted, but remains intact so that
Oct1 still binds...
Which of the following statements about Huntington's is true? You can retry this question, the average of your two answers is recorded. You should see feedback if you get a question wrong. O a. The mutated allele(s) has/have lost the function of keeping brain cells healthy, and is/are therefor a dominant disease O b. Huntington is a very rare and therfor recessive. c. Huntington strikes people after reproductive age and is therefor dominant. d. The mutated allele(s) has/have gained the...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
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