Homework Answers
Lac Z, Y, A are structural genes, which are transcribed as single m-RNA from Lac operon
Lac Z codes for beta-galactosidase enzyme, essential for digestion of lactose (type of beta-galactoside)
Lac Y gene codes for beta-galactoside permease, which transports beta-galactoside (lactose) in the cell of bacteria.
Thus mutation in Lac Z and Lac Y will have Digestion of lactose and production of beta-galactosidase will be inhibited.
Lac repressor is coded by Lac I, which prevents expression of lac ZYA by binding to operator, which inhibits RNA polymerase from transcribing lac ZYA. Lac I is transcribed by another promoter and is not controlled by availability of lactose in medium. Since mutation is not present in operator region and Lac I, the binding of repressor to operator will be unaffected.
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Mutations are introduced to the lacZ and lacy genes. Which of the following! most accurately describes...
The lac operon contains three genes: lacZ, lacY, and lacA for β‑galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively.
The lac operon contains three genes: lacZ, lacY, and lacA for β‑galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively. The operon also contains a promoter site and an operator site. The regulatory gene includes the I gene for the Lac repressor protein and a promoter for that gene.The lac operon undergoes negative regulation. In the normal condition, the Lac repressor protein is active. Allolactose, an isomer of lactose, is the signal molecule that binds to the Lac repressor. Determine which events...
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
A bacterium has the following genotype: lacIS lacP+ lacO- lacZ+ lacY- / lacI- lacP- lacO+ lacZ-...
A bacterium has the following genotype: lacIS lacP+ lacO- lacZ+ lacY- / lacI- lacP- lacO+ lacZ- lacY+ Fill in the blanks with either “on” or “off”. a. In the presence of lactose, transcription of β-galactosidase is __ON____. b. In the absence of lactose, transcription of β-galactosidase is ___ON___. c. In the presence of lactose, transcription of permease is __OFF____. d. In the absence of lactose, transcription of permease is __OFF____. Please give a detailed explanation....the mutations such as lacI^s are...
which operon will make b-galactosidase even if lactose isn't present? p+ o+ lacZ+ lacI- p- Oc...
which operon will make b-galactosidase even if lactose
isn't present?
p+ o+ lacZ+ lacI-
p- Oc lacZ+ lacI-
p+ Oc lacZ- lacI+
p- o+ lacZ+ lacI+
p+ o+ lacZ+ lacI+
Oc = fails to bind R
regulatory promoter operator gene structural genes lacz lacY lacA β-galactosidase thiogalactoside transacetylase lactose permease
UT EID: D. 5 E. 6 13. For the E. coli strain containing the following alleles...
UT EID: D. 5 E. 6 13. For the E. coli strain containing the following alleles of the lac operon, expression of lacZ and lacY is inducible, constitutively on or permanently repressed. (erepressor; r cannot bind operator: osoperator, o cannot bind repressor, lac are LOF mutations) A. lacZ is inducible, lacY is constitutively on. B. lacZ is constitutively on, lacY is inducible. C. lacZ is permanently repressed, lacY is constitutively on. D. lacZ is constitutively on, lacY is permanently repressed....
help please as soon as possible A mutant E. coli strain is found that does not...
help please as soon as possible
A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose (or allolactose). W mutation(s) can lead to this outcome? The lac operon is shown here as a guide. fones where proteins bind DNA genes and regulatory sequences Operator La repressor B-galactosidase B-galactos de transactylase proteins promoter Operator Lacy Lac A laci o Lacz
Background: Several genetic regions are needed for lactose metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene), lacY(permease gene), lacA(transacetylase...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions,...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
which operon will make b-galactosidase even if lactose
isn't present?
p+ o+ lacZ+ lacI-
p- Oc lacZ+ lacI-
p+ Oc lacZ- lacI+
p- o+ lacZ+ lacI+
p+ o+ lacZ+ lacI+
Oc = fails to bind R
regulatory promoter operator gene structural genes lacz lacY lacA β-galactosidase thiogalactoside transacetylase lactose permease
UT EID: D. 5 E. 6 13. For the E. coli strain containing the following alleles of the lac operon, expression of lacZ and lacY is inducible, constitutively on or permanently repressed. (erepressor; r cannot bind operator: osoperator, o cannot bind repressor, lac are LOF mutations) A. lacZ is inducible, lacY is constitutively on. B. lacZ is constitutively on, lacY is inducible. C. lacZ is permanently repressed, lacY is constitutively on. D. lacZ is constitutively on, lacY is permanently repressed....
help please as soon as possible
A mutant E. coli strain is found that does not synthesize any enzymes in the presence or absence of lactose (or allolactose). W mutation(s) can lead to this outcome? The lac operon is shown here as a guide. fones where proteins bind DNA genes and regulatory sequences Operator La repressor B-galactosidase B-galactos de transactylase proteins promoter Operator Lacy Lac A laci o Lacz
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
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