The answers are given in the above image as high or low please follow it.
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A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
Microbiology question 51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for lactose metabolism. The genotypes of different E. coli strains are listed below. (For this exercise, lacP refers to the promoter, and lacO refers to the operator site where Lacl binds. lacl+ means wild-type, whereas lacl- indicates a loss-of-function mutation. lacO- means that Lacl cannot bind the operator.) For each strain, indicate whether the LacZ protein will be synthesized under the conditions indicated, when glucose...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
B2. Consider E. coli cells, each having one of the following mutations: a) a mutant lac operator (Oc locus) that cannot bind repressor. b) a mutant lac repressor (I- gene product) that cannot bind to the lac operator. c) a mutant lac repressor (the Is gene product) that cannot bind to lactose. d) a mutant lac promoter that cannot bind CAP + cAMP. What effect would each mutation have on the function of lac operon (assuming no glucose is present)...
Imagine you are carrying out research on the lac operon. You isolate six mutations in the lac operon by measuring the amount of beta-galactosidase made in mutant cell line under three different conditions: no lactose/no glucose; lactose only; and lactose/glucose. Your results are shown in the table. Strain No Lactose, No Glucose Lactose Lactose, Glucose Wild-type None High Low Mutant 1 None None None Mutant 2 None None None Mutant 3 None Low Low Mutant 4 None Low Low Mutant...
gene regulation of bacteria operon systems. Protected View . Saved to this PG References Mailings Review View Help Tell me what you want to do ernet can contain viruses. Unless you need to edit, it's safe to stay in Protected View Enable Editing 5. What happens if lactose levels are low? Put the following list in order (1-5). RNA polymerase is blocked from transcribing the genes for the lactose metabolizing enzymes When RNA polymerase binds to the promoter, it cannot...
A number of mutations affect the expression of the lactose operon in E. coli. Complete the table given below. Use a + to indicate that the enzyme is synthesized at greater than basal levels, and a 0 to indicate that the enzyme is not synthesized. Assume glucose is NOT present. Lactose Present B-galactosidase Permease Lactose Absent B-galactosidase Permease (a) I+O+Z+Y+ (b) 1 O+Z+Y+ (c) 1-O+Z+Y+ (d) I+0 Z+Y+ (e) I OʻZ+Y- (f) I+0 Z+Y-/ F'1-O+Z- Y+ (g) I+P+O+Z-Y+/ F' I+P-OZ+Y-...
the answer I gave was wrong A mutant E. coli strain is found that synthesizes B-galactosidase whether or not lactose (or allolactose) is present. What mutations can independently lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences lac 1 operator promoter -35 lac z lac Y lac A JOSSA expression Lac repressor B-galactosidase B-galactoside- transacetylase proteins permease Lac Z Lac A promoter operator laci Lac Y
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
The lac operon consists of a promoter that initiates transcription of the gene i, which encodes a repressor protein. A seperate promoter, p, within the operon initiates transcription of z, which encodes B-galactosidase, and y, which encodes permease. The operator, o, where the repressor protein binds to inhibit transcription, is positioned between mutations, whereas superscript c denotes a constitutively active mutant. The table below sea fives the activity of B-galatosidase and permease for haploid and diploid mutant strains. Match the...