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A number of mutations affect the expression of the lactose operon in E. coli. Complete the...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
1. The map of the lac operon is P-O-Z-Y. The promoter (P) region is the start...
1. The map of the lac operon is P-O-Z-Y. The promoter (P) region is the start site of transcription through the binding of the RNA polymerase molecule before actual mRNA production. Mutationally altered promoters (P-) apparently cannot bind the RNA polymerase molecule. Certain predictions can be made about the effect of p-mutations, Use your knowledge of the lactose system to complete the following table. Insert a "4" where an enzyme is produced and a "_" where no enzyme is produced....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes:...
When attempting a targeted gene knockout using mouse embryonic stem cells, there are three possible outcomes: targeted knockout, ectopic insertion, and no insertion. What procedures can be used to select for cells that only have the targeted gene knockout? (9 pts) Describe why Jacob and Monod used IPTG as a synthetic inducer during their experiments investigating the genetic control of the lac operon. (6 pts) Describe the function of the CAP-cAMP system in bacteria. Why does it regulate several operons...
6. (2) What would be the expected expression of B-galactosidase in the E. coli strains listed...
6. (2) What would be the expected expression of B-galactosidase in the E. coli strains listed below? If the enzyme is not expressed indicate this with a "0" and if it is expressed over basal levels indicate this with a "+". Partially diploid genotypes represent F' (lac) strains. Lactose absent Lactose present Genotype HP+ O+ Z+ FP+ O+ 7+ P+ OC Z+ FP+ OC Z+ + pt o Z # P+ 0+ 7 7 P+ O Z/H P+ Of Z+...
PLEASE note that there may be (most likely be) more than one number that satisfies the...
PLEASE note that there may be (most likely be) more than one number that satisfies the answer. For example, for the first question, #1 may not be the only answer. PLEASE LIST ALL NUMBER THAT CORRESPONDS ISP+OCZ+Y+ For this genotype, indicate whether the enzyme is indicated is produced at significant levels. 1 ?-galactosidase, no lactose present 2 ?-galactosidase, lactose present as sole carbon source 3 permease, no lactose present 4 permease, lactose present as sole carbon source To enter your...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence or absence of lactose, as indicated below. The presence (+) or absence (-) of β-galactosidase and permease expression are investigated in these cultures. Which of the following options (A-E) would be the expected results for bacteria with the genotype IS P+ O+ Z+ Y- / I+ P+ OC Z– Y+ ? β-galactosidase Permease No lactose Lactose No lactose Lactose A - + - +...
PLEASE EXPLAIN ANSWERS Complete the table below using to indicate if the enzyme is synthesized and...
PLEASE EXPLAIN ANSWERS
Complete the table below using to indicate if the enzyme is synthesized and to indicate if the enzyme will not be synthesized. Genotype symbols: Ilacl (the repressor gene): P=Pac (the promoter), O = laco (the operator). Z = lacZ (the B-galactosidase gene), and Y lacy (the permease gene). The minus sign accompanying the symbol (0.g. 1) indicates a mutant gene, the plus sign indicates wild-type/functional gene. O is the constituitive operator mutation (the same as O-) and...
E. coli/brings lactose into its cell by using an enzyme called Lac Permease. Once the lactose...
E. coli/brings lactose into its cell by using an enzyme called Lac Permease. Once the lactose is inside, another enzyme called B-galactosidase converts the lactose into galactose and glucose. 1. How is E. coll's way of dealing with lactose similar to how your celis do it? 2. How is it different?
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
1. The map of the lac operon is P-O-Z-Y. The promoter (P) region is the start site of transcription through the binding of the RNA polymerase molecule before actual mRNA production. Mutationally altered promoters (P-) apparently cannot bind the RNA polymerase molecule. Certain predictions can be made about the effect of p-mutations, Use your knowledge of the lactose system to complete the following table. Insert a "4" where an enzyme is produced and a "_" where no enzyme is produced....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
6. (2) What would be the expected expression of B-galactosidase in the E. coli strains listed below? If the enzyme is not expressed indicate this with a "0" and if it is expressed over basal levels indicate this with a "+". Partially diploid genotypes represent F' (lac) strains. Lactose absent Lactose present Genotype HP+ O+ Z+ FP+ O+ 7+ P+ OC Z+ FP+ OC Z+ + pt o Z # P+ 0+ 7 7 P+ O Z/H P+ Of Z+...
PLEASE EXPLAIN ANSWERS
Complete the table below using to indicate if the enzyme is synthesized and to indicate if the enzyme will not be synthesized. Genotype symbols: Ilacl (the repressor gene): P=Pac (the promoter), O = laco (the operator). Z = lacZ (the B-galactosidase gene), and Y lacy (the permease gene). The minus sign accompanying the symbol (0.g. 1) indicates a mutant gene, the plus sign indicates wild-type/functional gene. O is the constituitive operator mutation (the same as O-) and...
E. coli/brings lactose into its cell by using an enzyme called Lac Permease. Once the lactose is inside, another enzyme called B-galactosidase converts the lactose into galactose and glucose. 1. How is E. coll's way of dealing with lactose similar to how your celis do it? 2. How is it different?
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