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6. (2) What would be the expected expression of B-galactosidase in the E. coli strains listed...
The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
The genotypes of the lac operon of several haploid and partial diploid E. coli strains are...
The genotypes of the lac operon of several haploid and
partial diploid E. coli strains are given. For each genotype,
indicate the status of B-galactosidase synthesis as either Yes or
No. Assume that no glucose is present and cAMP is rich in the
growth medium.
• Oc is a mutant operator that cannot be bound
by the wild type repressor protein.
• Is is a mutant of repressor gene and cannot
bind to operator
Haploid Partial Diploid IOZY IOCZY ISOZY...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
A number of mutations affect the expression of the lactose operon in E. coli. Complete the...
A number of mutations affect the expression of the lactose operon in E. coli. Complete the table given below. Use a + to indicate that the enzyme is synthesized at greater than basal levels, and a 0 to indicate that the enzyme is not synthesized. Assume glucose is NOT present. Lactose Present B-galactosidase Permease Lactose Absent B-galactosidase Permease (a) I+O+Z+Y+ (b) 1 O+Z+Y+ (c) 1-O+Z+Y+ (d) I+0 Z+Y+ (e) I OʻZ+Y- (f) I+0 Z+Y-/ F'1-O+Z- Y+ (g) I+P+O+Z-Y+/ F' I+P-OZ+Y-...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Absent in both...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Absent in both the presence and absence of lactose; (B) Abundant only in the absence of lactose; (C) Abundant only in the presence of lactose; (D) Abundant in both the presence and absence of lactose; or (E) Abundant only in the presence of glucose. I. i(-); II. p(-)o(c); III. i(-)p(+) / i(+)p(-); IV. i(s)p(+)o(c) / i(+)p(-)o(+).
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the F' plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the F' segment...
Consider the following partial diploid genotype of an Escherichia coli strain regarding the lac operon: lact...
Consider the following partial diploid genotype of an Escherichia coli strain regarding the lac operon: lact P+O+Z+Y+ A+/lacp+O+Z+Y+ A+ In the PRESENCE of lactose, what is the state of beta- galactosidase expression? O A.- OB. + OC. Can't tell
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
The genotypes of the lac operon of several haploid and
partial diploid E. coli strains are given. For each genotype,
indicate the status of B-galactosidase synthesis as either Yes or
No. Assume that no glucose is present and cAMP is rich in the
growth medium.
• Oc is a mutant operator that cannot be bound
by the wild type repressor protein.
• Is is a mutant of repressor gene and cannot
bind to operator
Haploid Partial Diploid IOZY IOCZY ISOZY...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
A number of mutations affect the expression of the lactose operon in E. coli. Complete the table given below. Use a + to indicate that the enzyme is synthesized at greater than basal levels, and a 0 to indicate that the enzyme is not synthesized. Assume glucose is NOT present. Lactose Present B-galactosidase Permease Lactose Absent B-galactosidase Permease (a) I+O+Z+Y+ (b) 1 O+Z+Y+ (c) 1-O+Z+Y+ (d) I+0 Z+Y+ (e) I OʻZ+Y- (f) I+0 Z+Y-/ F'1-O+Z- Y+ (g) I+P+O+Z-Y+/ F' I+P-OZ+Y-...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
Consider the following partial diploid genotype of an Escherichia coli strain regarding the lac operon: lact P+O+Z+Y+ A+/lacp+O+Z+Y+ A+ In the PRESENCE of lactose, what is the state of beta- galactosidase expression? O A.- OB. + OC. Can't tell
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
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