Note whether in the following strains of E. coli, B-galactosidase will be: (A) Absent in both...
Note whether in the following strains of E. coli, B-galactosidase will be:
(A) Absent in both the presence and absence of lactose;
(B) Abundant only in the absence of lactose;
(C) Abundant only in the presence of lactose;
(D) Abundant in both the presence and absence of lactose; or
(E) Abundant only in the presence of glucose.
I. i(-);
II. p(-)o(c);
III. i(-)p(+) / i(+)p(-);
IV. i(s)p(+)o(c) / i(+)p(-)o(+).
Homework Answers
I. i(-) : (D) Abundant in both the presence and absence of lactose.
Since it is lac i -, no repressor will be formed to bind the operator,so beta galactosidase will be formed both in presence and absence of lactose.
II. p(-)o(c): (A) Absent in both the presence and absence of lactose;
Constitutive operator will not allow repressor to bind but as promoter is absent/mutated , no beta galactosidase will be formed in presence or absence of lactose.
III. i(-)p(+) / i(+)p(-): (C) Abundant only in the presence of lactose
In presence of lactose only,beta galactosodase will be formed as repressor fails to bind to operator.
IV. i(s)p(+)o(c) / i(+)p(-)o(+): (D) Abundant in both the presence and absence of lactose
In this case, always beta galactosidase will be formed in presence or absence of lactose.
Add Answer to:
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Absent in both...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
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Three different strains of E. coli carry a mutation in the lac operon and/or laci gene....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
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A) Given the following genotypes of E. coli strains, predict whether the lacZ gene will be...
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genetics An E. Coli strain possesses the following genotype: F(P+)/" pCo+z+y+ where l' is a mutation...
genetics
An E. Coli strain possesses the following genotype: F(P+)/" pCo+z+y+ where l' is a mutation that eliminates the production of repressor and PC eliminates the ability of the polymerase to bind the promoter. The structural genes will be transcribed both in the presence and absence of lactose O will be transcribed only if lactose is absent O will be transcribed only if lactose is present will not be transcribed under any onditions will be transcribed but not translated
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
6. (2) What would be the expected expression of B-galactosidase in the E. coli strains listed below? If the enzyme is not expressed indicate this with a "0" and if it is expressed over basal levels indicate this with a "+". Partially diploid genotypes represent F' (lac) strains. Lactose absent Lactose present Genotype HP+ O+ Z+ FP+ O+ 7+ P+ OC Z+ FP+ OC Z+ + pt o Z # P+ 0+ 7 7 P+ O Z/H P+ Of Z+...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
13. The rate of B-galactosidase synthesis in E. coli is influenced by the bacteria's genotype and its growth conditions. Indicate whether you would expect the rate of β-galactosidase synthesis to increase, decrease, or remain the same after each of the following changes to the growth medium. Explain your answer. a. E. coli cells are cultured in minimal medium containing glycerol as the sole b. E. coli cells are cultured in minimal medium containing glucose as the sole c. E. coli...
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A number of mutations affect the expression of the lactose operon in E. coli. Complete the table given below. Use a + to indicate that the enzyme is synthesized at greater than basal levels, and a 0 to indicate that the enzyme is not synthesized. Assume glucose is NOT present. Lactose Present B-galactosidase Permease Lactose Absent B-galactosidase Permease (a) I+O+Z+Y+ (b) 1 O+Z+Y+ (c) 1-O+Z+Y+ (d) I+0 Z+Y+ (e) I OʻZ+Y- (f) I+0 Z+Y-/ F'1-O+Z- Y+ (g) I+P+O+Z-Y+/ F' I+P-OZ+Y-...
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