Homework Answers
The enzyme can hydrolyze lactose to galactose plus glucose, it can transgalactosylate to form allolactose, and it can hydrolize allolactose. The presence of lactose results in the synthesis of allolactose which bings to the lac repressor and reduce its affinity for the lac operon. Thus the answer is A-
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Consider the following partial diploid genotype of an Escherichia coli strain regarding the lac operon: lact...
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what...
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what is the status of beta- galactosidase activity in the PRESENCE of lactose? O A. - OB. + c. Can't tell
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the F' plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the F' segment...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
The genotypes of the lac operon of several haploid and partial diploid E. coli strains are...
The genotypes of the lac operon of several haploid and
partial diploid E. coli strains are given. For each genotype,
indicate the status of B-galactosidase synthesis as either Yes or
No. Assume that no glucose is present and cAMP is rich in the
growth medium.
• Oc is a mutant operator that cannot be bound
by the wild type repressor protein.
• Is is a mutant of repressor gene and cannot
bind to operator
Haploid Partial Diploid IOZY IOCZY ISOZY...
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region...
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E, coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/). The possible answers indicate with a"+" or awhether beta-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence or absence of lactose, as indicated below. The presence (+) or absence (-) of β-galactosidase and permease expression are investigated in these cultures. Which of the following options (A-E) would be the expected results for bacteria with the genotype IS P+ O+ Z+ Y- / I+ P+ OC Z– Y+ ? β-galactosidase Permease No lactose Lactose No lactose Lactose A - + - +...
The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
Can you please help me with this problem: 4. Suppose the lac operon partial diploid cap...
Can you please help me with this problem:
4. Suppose the lac operon partial diploid cap 1 P O T Y* / capt r pt O* 7' r is grown. Note: cap affects the CAP binding site. a. Will this partial diploid strain grow on a lactose medium? Explain. (2 pts) b. Is transcription of B-galactosidase and permease inducible, constitutive, or noninducible? Explain. (2 pts) c. Explain how genetic complementation contributes to the growth habit of this strain. (2 pts)
Determine the outcome for the lac operon genotype shown below: Assume lactose is absent. Select one:...
Determine the outcome for the lac operon genotype shown below: Assume lactose is absent. Select one: o Functional beta-galactosidase and functional permease are produced O Only functional beta-galactosidase is produced. Functional permease is NOT produced. O Only functional permease is produced. Functional beta-galactosidase is NOT produced. O Functional beta-galactosidase is NOT produced. Functional permease is NOT produced. Determine the outcome for the lac operon genotype shown below: SD oc Assume lactose is absent. Select one: o Functional beta-galactosidase and functional...
4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str S i+...
4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str S i+ o+ z+ y+ (that you grew in the absence of lactose) with a lac - F - strain with the genotype str R i- o+ z - y. You allow sufficient time for the transfer of the genes indicated, with the exception of str S , and plate the bacteria on minimal medium supplemented with arabinose and streptomycin but no lactose. You observe transient...
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what is the status of beta- galactosidase activity in the PRESENCE of lactose? O A. - OB. + c. Can't tell
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
The genotypes of the lac operon of several haploid and
partial diploid E. coli strains are given. For each genotype,
indicate the status of B-galactosidase synthesis as either Yes or
No. Assume that no glucose is present and cAMP is rich in the
growth medium.
• Oc is a mutant operator that cannot be bound
by the wild type repressor protein.
• Is is a mutant of repressor gene and cannot
bind to operator
Haploid Partial Diploid IOZY IOCZY ISOZY...
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E, coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/). The possible answers indicate with a"+" or awhether beta-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
Can you please help me with this problem:
4. Suppose the lac operon partial diploid cap 1 P O T Y* / capt r pt O* 7' r is grown. Note: cap affects the CAP binding site. a. Will this partial diploid strain grow on a lactose medium? Explain. (2 pts) b. Is transcription of B-galactosidase and permease inducible, constitutive, or noninducible? Explain. (2 pts) c. Explain how genetic complementation contributes to the growth habit of this strain. (2 pts)
Determine the outcome for the lac operon genotype shown below: Assume lactose is absent. Select one: o Functional beta-galactosidase and functional permease are produced O Only functional beta-galactosidase is produced. Functional permease is NOT produced. O Only functional permease is produced. Functional beta-galactosidase is NOT produced. O Functional beta-galactosidase is NOT produced. Functional permease is NOT produced. Determine the outcome for the lac operon genotype shown below: SD oc Assume lactose is absent. Select one: o Functional beta-galactosidase and functional...
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