Homework Answers
IS mutations change the repressor so that it can no longer bind the inducer. lac IS mutant is dominant, trans acting and uninducible.
these are known as superrepressed mutations. when studied in vitro, the mutant is polypeptides form tetramers that bind to lac operator DNA. they either donot bind inducer, or exhibit a very low affinity for inducer. the is mutations therefore modify the inducer binding siteof the lac repressor.
hence, the inducer cannot bind. no transcription and translation and thus no products.
there would be no products even in presence or absence of lactose.
Oc mutations are constitutive mutations. they act only in cis, i.e, they cause constitutive expression of structural genes present on the same chromosome.
ques 1: option C. only permease is produced. beta galactosidase is not produced.
ques 2: option A. both the enzymes are produced.
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Determine the outcome for the lac operon genotype shown below: Assume lactose is absent. Select one:...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
Determine the outcome for the lac operon genotype shown below: I-P+O+Z+Y-/I+P+OcZ-Y+ Assume lactose is present.
Determine the outcome for the lac operon genotype shown below: I-P+O+Z+Y-/I+P+OcZ-Y+ Assume lactose is present.
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the F' plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the F' segment...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene....
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence...
Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence or absence of lactose, as indicated below. The presence (+) or absence (-) of β-galactosidase and permease expression are investigated in these cultures. Which of the following options (A-E) would be the expected results for bacteria with the genotype IS P+ O+ Z+ Y- / I+ P+ OC Z– Y+ ? β-galactosidase Permease No lactose Lactose No lactose Lactose A - + - +...
Consider the following partial diploid genotype of an Escherichia coli strain regarding the lac operon: lact...
Consider the following partial diploid genotype of an Escherichia coli strain regarding the lac operon: lact P+O+Z+Y+ A+/lacp+O+Z+Y+ A+ In the PRESENCE of lactose, what is the state of beta- galactosidase expression? O A.- OB. + OC. Can't tell
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what...
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what is the status of beta- galactosidase activity in the PRESENCE of lactose? O A. - OB. + c. Can't tell
For each region of the lac operon on the F' plasmid, I+ P+ Oc Z− Y+...
For each region of the lac operon on the F' plasmid, I+ P+ Oc Z− Y+ , determine whether the region is wild type (that is, it produces a functional protein or it's a correct protein binding sequence) or whether the region is mutated. Select all that apply. Select all that apply. The promoter sequence is correct/functional. The repressor protein is produced. The operator sequence is correct/functional. Beta-galactosidase is produced from the lacZ gene. Permease is produced from the lacY...
The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
Three different strains of E. coli carry a mutation in the lac operon and/or laci gene. The production of B-galactosidase (+ present or - absent) is measured when lactose is present and absent from the medium. Assume the mutations involve only 1, 0, or Z. A merodiploid is constructed for each of the three strains. The plasmid carries a wild type lac operon and lacl gene. The production of functional B-galactosidase (+ present or - absent) is measured when lactose...
Consider the following partial diploid genotype of an Escherichia coli strain regarding the lac operon: lact P+O+Z+Y+ A+/lacp+O+Z+Y+ A+ In the PRESENCE of lactose, what is the state of beta- galactosidase expression? O A.- OB. + OC. Can't tell
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what is the status of beta- galactosidase activity in the PRESENCE of lactose? O A. - OB. + c. Can't tell
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
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