Determine the outcome for the lac operon genotype shown below:
I-P+O+Z+Y-/I+P+OcZ-Y+
Assume lactose is present.
since lacI gene producing repressor protein so it will bind to the operator and inhibit the transcription of lac operon. and in the question lactose is also present, so lactose will bind to the repressor protein and inactivates it. because of inactivation, it will no longer be able to bind to the operator sequence, so RNA polymerase will able to bind to the promoter region and start the transcription of lac operon.
Determine the outcome for the lac operon genotype shown below: I-P+O+Z+Y-/I+P+OcZ-Y+ Assume lactose is present.
Determine the outcome for the lac operon genotype shown below: Assume lactose is absent. Select one: o Functional beta-galactosidase and functional permease are produced O Only functional beta-galactosidase is produced. Functional permease is NOT produced. O Only functional permease is produced. Functional beta-galactosidase is NOT produced. O Functional beta-galactosidase is NOT produced. Functional permease is NOT produced. Determine the outcome for the lac operon genotype shown below: SD oc Assume lactose is absent. Select one: o Functional beta-galactosidase and functional...
You plate colonies of E. coli with the lac operon genotype of I+P+OCZ+CAP+ on 1) a medium containing only glucose, and 2) a medium containing only lactose. You isolate mRNA from bacteria from each medium and do a Northern blot with a probe for β-galactosidase. Draw the results.
The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the product of the lacZ gene. The diploid conditions represent the addition of a plasmid carrying different components of the lac operon. Determine if β-gal will be generated under the conditions. Assume that glucose is absent. A + in the genotype indicates a functioning gene, while a – indicates a loss-of-function allele. The OC is an operator mutant that cannot bind the lacI protein. Use...
There are two different strains of E. coli. One type is a wild type and the other is a lac operon mutant. The lac operon wild type has the genotype: I+P+O+Z+Y+A+ What is a silent mutation? What would be the result for the lac operon genes?
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the F' plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the F' segment...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a mutant lac repressor that cannot bind the lac operator I^s a mutant lac repressor that cannot bind lactose O^c a mutant lac operator that cannot bind repressor Lacl P^- a mutant lac promoter that cannot bind RNA polymerase Z^- a mutant lacZ lost beta-galactosidase activity Y^- a mutant permease that cannot transport lactose into the cell Consider the following strains each with the indicated...
Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following statements is consistent with the expression phenotype? genome: I+P+ O- Z+ Y- plasmid: I+ P- O+ Z- Y+ constitutive expression of β-gal and permease induced in the presence of lactose constitutive expression of β-gal only constitutive expression of β-gal and permease β-gal and permease induced in the presence of lactose constitutive expression of permease and β-gal induced in the presence of lactose
Consider the following partial diploid genotype of an Escherichia coli strain regarding the lac operon: lact P+O+Z+Y+ A+/lacp+O+Z+Y+ A+ In the PRESENCE of lactose, what is the state of beta- galactosidase expression? O A.- OB. + OC. Can't tell
Consider the following lac operon genotype: laci+P+OCZY+A+ In an E. coli strain with this genotype, what is the status of beta- galactosidase activity in the PRESENCE of lactose? O A. - OB. + c. Can't tell