Question

Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following...

Lac Operon IPOZY v3 In the genotype presented (genomic plus plasmid genes), which of the following statements is consistent with the expression phenotype? genome: I+P+ O- Z+ Y- plasmid: I+ P- O+ Z- Y+

  • constitutive expression of β-gal and permease induced in the presence of lactose

  • constitutive expression of β-gal only

  • constitutive expression of β-gal and permease

  • β-gal and permease induced in the presence of lactose

  • constitutive expression of permease and β-gal induced in the presence of lactose

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Answer #1

1. Answer: Option 5 is correct
Explanation:
The Lac operon is a negatively regulated inducible operon.
It is expressed only in the presence of lactose provided that glucose is absent.
Genotype = I+P+O-Z+Y-/I+P-O+Z-Y+
Genomic copy = I+P+O-Z+Y-
Since it is O-, Z+ (Beta-gal) is expressed in the presence of lactose.

Plasmid copy = I+P-O+Z-Y+
Since it is P-, Y+ (Permease) is never expressed.

= Inducible expression of LacZ and constitutive repression of LacY


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