You are conducting an experiment using wild-type E. coli and you find that the beta-galactosidase activity...
You are conducting an experiment using wild-type E. coli and you find that the beta-galactosidase activity of a culture grown in glycerol minimal medium is the same as a culture grown in lactose minimal medium. Is this expected?
True or false:
Glycerol is another possible carbon source for E. coli and it is not either glucose or lactose.
Homework Answers
False. Recent studies shown that beta- galactosidase activity is high in minimal medium supplemented solely withglycerol and IPTG. Interestingly, it was found that beta-galactosidase induction abruptly decreases and remains suppressed when lactose is added to glycerol culture. This observation is counterintuitive, as one would expect higher beta-galactosidase activity in the presence of excess lactose.
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You are conducting an experiment using wild-type E.
coli and you find that the beta-galactosidase activity...
13. The rate of B-galactosidase synthesis in E. coli is influenced by the bacteria's genotype and...
13. The rate of B-galactosidase synthesis in E. coli is influenced by the bacteria's genotype and its growth conditions. Indicate whether you would expect the rate of β-galactosidase synthesis to increase, decrease, or remain the same after each of the following changes to the growth medium. Explain your answer. a. E. coli cells are cultured in minimal medium containing glycerol as the sole b. E. coli cells are cultured in minimal medium containing glucose as the sole c. E. coli...
You performed a ß-galactosidase assay of a wild-type strain that was grown in either minimal glucose,...
You performed a ß-galactosidase assay of a wild-type strain that was grown in either minimal glucose, glycerol or lactose media. Determine what media each of the following strains were grown in and explain your reasoning. Strain Miller Units 1 29 2 65 3 1088
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
Six E. colimutants were isolated. The activity of the enzyme beta-galactosidase produced by the cells was...
Six E. colimutants were isolated. The activity of the enzyme beta-galactosidase produced by the cells was measured when the cells were grown in medium supplemented with different carbon sources. Put your answer into the right-hand column of the table. Glycerol Lactose Lactose + Glucose Mutation in Wild-type 0 1000 10 -------- Mutant 1 0 10 10 Mutant 2 1000 1000 10 Mutant 3 0 0 0 Mutant 4 0 1000 10 Mutant 5 1000 1000 10 Mutant 6 0 0...
The genotypes of the lac operon of several haploid and partial diploid E. coli strains are...
The genotypes of the lac operon of several haploid and
partial diploid E. coli strains are given. For each genotype,
indicate the status of B-galactosidase synthesis as either Yes or
No. Assume that no glucose is present and cAMP is rich in the
growth medium.
• Oc is a mutant operator that cannot be bound
by the wild type repressor protein.
• Is is a mutant of repressor gene and cannot
bind to operator
Haploid Partial Diploid IOZY IOCZY ISOZY...
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region...
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E, coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/). The possible answers indicate with a"+" or awhether beta-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in...
Note whether in the following strains of E. coli, B-galactosidase will be: (A) Abundant in both...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
Please solve all of them Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP...
Please solve all of them
Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP acolacZacY Carbon source in theLac operon p-galactosidaseLactose permease binding site me levels in levels in the cell polycistronic mRNA enzy levels in the cell Undetectable Low but detectable Low but deteteable Low but detectable medium the cell Undetectable membrane Glucose only Glucose and lactose Lactose on Another carbon source Undetectable Undetectable Undetectable Undetectable The table shows the results of experiments measuring expression of the...
please help answer, thank you! In this eight-year experiment, 12 populations of E. coli, each begun...
please help answer, thank you!
In this eight-year experiment, 12 populations of E. coli, each begun from a single cell, were grown in low- glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the populations, and their fitness in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population. Which of the following, if it occurs in the absence of any...
Q2 An E. coli culture growing on a lactose supplemented media does not produce any β-galactosidase....
Q2 An E. coli culture growing on a lactose supplemented media does not produce any β-galactosidase. Identify the incorrect statement. a. The E. coli could be a lacZ mutant b. The E. coli may have a mutated LacI protein that cannot bind to the operator c. The E. coli may have a mutated LacI protein that cannot bind to the inducer d. The growth medium may have a high amount of glucose e. The E. coli may have a mutated...
13. The rate of B-galactosidase synthesis in E. coli is influenced by the bacteria's genotype and its growth conditions. Indicate whether you would expect the rate of β-galactosidase synthesis to increase, decrease, or remain the same after each of the following changes to the growth medium. Explain your answer. a. E. coli cells are cultured in minimal medium containing glycerol as the sole b. E. coli cells are cultured in minimal medium containing glucose as the sole c. E. coli...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
The genotypes of the lac operon of several haploid and
partial diploid E. coli strains are given. For each genotype,
indicate the status of B-galactosidase synthesis as either Yes or
No. Assume that no glucose is present and cAMP is rich in the
growth medium.
• Oc is a mutant operator that cannot be bound
by the wild type repressor protein.
• Is is a mutant of repressor gene and cannot
bind to operator
Haploid Partial Diploid IOZY IOCZY ISOZY...
The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E, coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/). The possible answers indicate with a"+" or awhether beta-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in...
Note whether in the following strains of E. coli,
B-galactosidase will be:
(A) Abundant in both the presence and absence of lactose
(B) Abundant only in the absence of lactose
(C) Abundant only in the presence of lactose
(D) Abundant only in the presence of sucrose
(D) Absent in both the presence and absence of lactose
Can you answer and explain these please?
What is the correct gene order and which 2 genes are most closely linked? (4 Points) o+at...
Please solve all of them
Lac Mutants 1-Copy 2 of 10 CAP laclCAP gene lacO lacP acolacZacY Carbon source in theLac operon p-galactosidaseLactose permease binding site me levels in levels in the cell polycistronic mRNA enzy levels in the cell Undetectable Low but detectable Low but deteteable Low but detectable medium the cell Undetectable membrane Glucose only Glucose and lactose Lactose on Another carbon source Undetectable Undetectable Undetectable Undetectable The table shows the results of experiments measuring expression of the...
please help answer, thank you!
In this eight-year experiment, 12 populations of E. coli, each begun from a single cell, were grown in low- glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the populations, and their fitness in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population. Which of the following, if it occurs in the absence of any...
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