You performed a ß-galactosidase assay of a wild-type strain that was grown in either minimal glucose,...
You performed a ß-galactosidase assay of a wild-type strain that was grown in either minimal glucose, glycerol or lactose media. Determine what media each of the following strains were grown in and explain your reasoning.
| Strain | Miller Units |
| 1 | 29 |
| 2 | 65 |
| 3 | 1088 |
Homework Answers
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You performed a ß-galactosidase assay of a wild-type strain that
was grown in either minimal glucose,...
You are conducting an experiment using wild-type E. coli and you find that the beta-galactosidase activity...
You are conducting an experiment using wild-type E. coli and you find that the beta-galactosidase activity of a culture grown in glycerol minimal medium is the same as a culture grown in lactose minimal medium. Is this expected? True or false: Glycerol is another possible carbon source for E. coli and it is not either glucose or lactose.
A. Which strain is the wild-type E.coli? Explain how you know this. B. Which strain contains...
A. Which strain is the wild-type E.coli? Explain how you know
this.
B. Which strain contains the nonsense mutation in the CRP
protein gene? Explain how you know this.
C. Which strain contains the deletion mutation in the lac operon
operator sequence? Explain how you know this.
knowledge about the lac operon system AND knowledge Problem set about the Lac operon (these problems require knowledge about the lac operon system AND about consequences of mutations) You are working with three...
Diploid strain genotypes With Galactose Without Galactose wild-type/wild-type AGAL4/AGAL4 MIG1* (Mig1 always in the nucleus)/wild-type AGAL8O/wild-type,...
Diploid strain genotypes With Galactose Without Galactose wild-type/wild-type AGAL4/AGAL4 MIG1* (Mig1 always in the nucleus)/wild-type AGAL8O/wild-type, AGAL4/wild-type AGAL80/ AGAL80 GAL3*/wild-type 9) In an attempt to better understand GAL gene regulation, an undergraduate researcher performed a series of mating experiments between haploid yeast strains that contained different mutations in the gal regulatory genes, generating the different diploid genotypes shown below (note, for each strain, only the changes, relative to wild-type, are indicated; assume that all other genes are wild-type). For each...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
You isolate 20 different OC mutant strains that are wild type for all other components of...
You isolate 20 different OC mutant strains that are wild type for all other components of the Lac Operon. You observe that when grown on lactose all of the strains exhibit a relative B-gal concentration of 100. When each strain is grown on glucose they all exhibit constitutive expression of B-gal, but the different strains exhibit different Bgal concentrations that range from 20 to 100. Which of the following could account for these observations? a) the OC mutant strains each...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-)...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-) and another yeast strain, which is His+Met+. One hundred asci were dissected, and colonies were grown from the four spores in each ascus. Cells from these colonies were tested for their ability to grow on petri plates containing either minimal medium(min), min+histidine (his), min+methionine (met),or min+his+met. The number of tetrads with four spores that can grow in different type of media is shown in...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild type strain of this bacteria can synthesize its own alanine (alat) and its own tryptophan trp' ), both of which are essential amino acids. A mutant strain of the same bacterium cannot synthesize either of those two amino acids (ala trp'). You are interested in the degree of linkage between the ala and the trp genes in this new bacterial species. Fortunately, this species...
Title: Development of an Adhesion Assay and Characterization of an Adhesion-Deficient Mutant of Pseudomonas fluorescens RESULTS...
Title: Development of an Adhesion Assay and Characterization of an Adhesion-Deficient Mutant of Pseudomonas fluorescens RESULTS Bacterial adhesion to sand columns. In the initial column assays with soil-and-sand mixtures, P. fluorescens PfO-1 and E. coli SLH25 had the highest and lowest adhesion values, respectively. Therefore, the sand column assay was optimized to yield the greatest difference in percent adhesion for these two strains. PfO-1 bacteria grown in minimal medium to the logarithmic phase were washed and suspended in either minimal...
You are given a mixed culture of two organisms in a small tube of TSB.
You are given a mixed culture of two organisms in a small tube of TSB. These two organisms include a Gram-negative strain (your bacterial strain to identify) and a Gram-positive strain (a contaminant). Your first task is to isolate these organisms which will help you identify which Gram-negative strain you are working with. 1) You inoculate two streak plates on TSA media and MacConkey Agar using aseptic technique. For each medium, state whether the medium is selective, differential, or neither and...
A. Which strain is the wild-type E.coli? Explain how you know
this.
B. Which strain contains the nonsense mutation in the CRP
protein gene? Explain how you know this.
C. Which strain contains the deletion mutation in the lac operon
operator sequence? Explain how you know this.
knowledge about the lac operon system AND knowledge Problem set about the Lac operon (these problems require knowledge about the lac operon system AND about consequences of mutations) You are working with three...
Diploid strain genotypes With Galactose Without Galactose wild-type/wild-type AGAL4/AGAL4 MIG1* (Mig1 always in the nucleus)/wild-type AGAL8O/wild-type, AGAL4/wild-type AGAL80/ AGAL80 GAL3*/wild-type 9) In an attempt to better understand GAL gene regulation, an undergraduate researcher performed a series of mating experiments between haploid yeast strains that contained different mutations in the gal regulatory genes, generating the different diploid genotypes shown below (note, for each strain, only the changes, relative to wild-type, are indicated; assume that all other genes are wild-type). For each...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
A cross was performed between a yeast strain that requires histidine and methionine for growth (His-Met-) and another yeast strain, which is His+Met+. One hundred asci were dissected, and colonies were grown from the four spores in each ascus. Cells from these colonies were tested for their ability to grow on petri plates containing either minimal medium(min), min+histidine (his), min+methionine (met),or min+his+met. The number of tetrads with four spores that can grow in different type of media is shown in...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild type strain of this bacteria can synthesize its own alanine (alat) and its own tryptophan trp' ), both of which are essential amino acids. A mutant strain of the same bacterium cannot synthesize either of those two amino acids (ala trp'). You are interested in the degree of linkage between the ala and the trp genes in this new bacterial species. Fortunately, this species...
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