Question

QUESTION 7 You recovered three suppressor mutants in the suppressor screen of HB1 yeast cells, which you labeled SUP1, SUP2, and SUP3. You performed the three tests as described in the lab manual, and the results are depicted below. What kind of mutation is SUP1? Control HA1 SUP1 Control HA1 HB1 HB2 SUP1 HA1 SUP1 SUP2 SUP3 Edns SUP3 SUP2 zans Petite selection medium plate Complementation test on YED plate Ade(-) minimal medium plate Adenosine biosynthesis pathway mutation, upstream of ADE2 Intragenic suppressor mutation Petite suppressor mutation The test is inconclusive and need to be repeated

Answer 1

Adenosine biosynthesis pathway mutation ,upstream of ADE2 is observed is suppresses the HB1 Yeast cells in the suppressing screen.SUP1 growth is not observed in the ade(-) minimal medium plate.