Question

define these terms using one word, few words, one sentence

Vocabulary/definitions (one word, few words, or one sentence answers) 1. Sl nuclease 2. DNase I 3. DNase footprint 4. EMSA/gel shift 5. 2 kinds of plasmids (slide 28) 6. 2 functional domains of GAL4 protein 7. CHIP 8. Bait-hybrid 9. Fish-hybrid 10.3 functional domains of steroid receptors

Answer 1

1.S1 Nuclease - Endonuclease, degrades single-stranded nucleic acids.

2.DNase1-  DNA specific Endonuclease that Removal of contaminating genomic DNA from RNAs.

3.DNase footprint - In vitro method for locating the specific binding sites of proteins on DNA.

4.EMSA/gel shift - Used to detect protein complexes with nucleic acids.

5.2 kinds of plasmid(slide 28) - make sure your question is correct,i think slide 28 in your book somewhere written,otherwise ,five main types of plasmids is F-plasmids, resistance plasmids, virulence plasmids, degradative plasmids, and Col plasmids.

6.2 functional domains of GAL 4 protein - DNA-Binding domain and the transcriptional Activation Domain.

7.CHIP - Chromatin immunoprecipitation (ChIP) assay is used for studing protein-DNA interactions among the natural chromatin context of the cell.

8.Bait- hybrid - identify and establish protein-protein interactions.

9.Fish hybrid - Two fish species to hybridise which need to be very closely related to gene pool.

10.3 functional domains of steroid receptors - DBD(DNA binding domain),LBD(Ligand-binding domain),NTD(Amino terminal domain)