6. What is the general approach to changing fluorescence spectrum of GFPs? explain briefly
GFPs is mainly used to monitor gene expressions. Utilizing the small fluorescent molecules is a powerful and attractive technique for in vivo and in vitro analysis of cellular proteins.
Some proteins shows initial increase in fluorescence intensity upon application of pressure above ambient conditions.
Small blue shifts in the fluorescence spectra with increasing pressure were seen in all proteins under study, hinting at increased rigidity of the chromophore environment. Thus changing fluorescence spectrum also changes the pressure, some exhibit strong and abrupt changes in their fluorescence spectra at certain pressures due to structural modifications of the hydrogen bonding environment of the chromophore. now for such proteins to indentify the clear 3 dimentional structures absorption and emission.
Fluorescence emission shows contributions from two chemical relaxation processes as well as diffusional concentration fluctuations. spectral properties changes due to irradiation with light of a specific wavelength and intensity
Some of the yellow-shifted GFP mutants that exhibit a dramatically decreased fluorescence quantum yield with fluorescence losses more than 90% and extremely short excited state lifetimes have been interpreted in terms of internal conversion induced by torsional motion within the chromophore which is all induced by the fluorescence spectrum.
Therefore it is neccessary to set the proper fluorescence spectrum for GFPs to understand absorption emmision and 3D structure of particular protein thereby understanding the fluorescent properties
6. What is the general approach to changing fluorescence spectrum of GFPs? explain briefly
Use the absorption and fluorescence spectra of bacteriochlorophyll a dissolved in toluene to estimate: (a) the Stokes shift of the fluorescence spectrum (b) the frequency (in cm−1) of the vibrational mode that is displaced (stretched) by the optical transition from the ground state to the excited state. Compare the estimates you get from the vibrational structure of the absorption and fluorescence spectra. What part of the bacteriochlorophyll macrocycle is likely to be changing structure?Explain. (c) the energy gaps in cm−1...
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5(15%) Shown below are the absorption and fluorescence spectra for an antibody in pH 72 buffer: 350 400 450 500 550 600 650 700 750 Wavelength (nm) a. Which is the fluorescence spectrum, the red one or the blue one? Explain. b. What is the best wavelength at which to excite this sample in order to study its fluorescence? Explain. c. What is the best wavelength at which to monitor the fluorescence of this sample for quantitative purposes? Explain. d....
Explain briefly The challenges and dilemmas related to prescribing and What are the general principles apply in relation to prescribing?
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Q1. Explain briefly The challenges and dilemmas related to prescribing? Q2. What are the general principles apply in relation to prescribing?
Question: Q. Explain briefly The challenges and dilemmas related to prescribing? Q2. What are the general principles apply in relation to prescribing?
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Is it possible to have a colony exhibiting ampicillin resistance and not fluorescence? Explain why or why not. in general and experiment based off E.coli and plamid pGFP