Is it possible to have a colony exhibiting ampicillin resistance and not fluorescence? Explain why or...
Is it possible to have a colony exhibiting ampicillin resistance and not fluorescence? Explain why or why not.
Homework Answers
Yes it is possible, due to following reasons :-
May be the gene on interest , is not inserted properly, it's partly inserted or only the ampicillin resistance gene portion is inserted and rest is not.
It can also be possible, that the products/ conditions necessary to fluorescent for example the substrate is not well made or provided, leading to false negatives.
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Is it possible to have a colony exhibiting ampicillin resistance
and not fluorescence? Explain why or...
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in...
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in the cloning experiment. (b) Sketch and explain what would occur if you plate your post-cloning specimens on (1) a plate with methicillin and (ii) ampicillin? Explain the results in your sketch.
Explain why patients exhibiting cystic fibrosis should have different NPD levels than patients no...
Explain why patients exhibiting cystic fibrosis should have different NPD levels than patients not exhibiting cystic fibrosis.
(Need to explain B. cereus resistance to penicillin and ampicillin. This was most likely because B....
(Need to explain B. cereus resistance to penicillin and ampicillin. This was most likely because B. cereus has been shown to possess β-lactamase (ref).)
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance...
You insert a gene for tetracycline resistance into one plasmid and a gene for ampicillin resistance into another plasmid. You successfully introduce both plasmids into a sample of E. coli cells, but fail to grow any of them in culture medium with both antibiotics present in it. What could best explain the problem? A. Random mutation has inactivated the antibiotic resistance genes B. Plasmid incompatibility will not allow both plasmids to persist C. E. coli cannot maintain two plasmids D....
Why is soil been chosen to study for antibiotic resistance with the PARE Project? Explain what...
Why is soil been chosen to study for antibiotic resistance with the PARE Project? Explain what is meant by the term ‘dilution factor’ in the PARE Project? Why is it important to select a well-isolated colony for use in species identification? 4.16S rRNA was chosen for species identification. Expand on why this gene is used.
Discuss why microorganisms often have different colony morphologies on different media?
Discuss why microorganisms often have different colony morphologies on different media?
Question 5 (2 marks) (a) Explain the relationship between UV-Vis Spectroscopy and Fluorescence using the Riboflavin...
Question 5 (2 marks) (a) Explain the relationship between UV-Vis Spectroscopy and Fluorescence using the Riboflavin molecule shown below: 0.025 0.02 0.015 absorption CH Ads 0.005 fluorescence LOH 250350450550650750850 OH (b) Why must a molecule that fluoresces also have an absorption spectrum?
Please dont forget energy level diagrams Using energy level diagrams explain why in fluorescence spectroscopy, the...
Please dont forget energy level diagrams
Using energy level diagrams explain why in fluorescence spectroscopy, the wavelength of the electromagnetic radiation emitted is longer than the wavelength absorbed.
is it possible to have a standard deviation of 435,000 for some days set? explain why...
is it possible to have a standard deviation of 435,000 for some days set? explain why or why not.
Decide if the following statement Is true or false. Explain why. It is possible to have...
Decide if the following statement Is true or false. Explain why. It is possible to have a standard deviation of 440, 000
4. During cloning we insert an ampicillin resistance gene a plasmid, (a) Explain its function in the cloning experiment. (b) Sketch and explain what would occur if you plate your post-cloning specimens on (1) a plate with methicillin and (ii) ampicillin? Explain the results in your sketch.
Question 5 (2 marks) (a) Explain the relationship between UV-Vis Spectroscopy and Fluorescence using the Riboflavin molecule shown below: 0.025 0.02 0.015 absorption CH Ads 0.005 fluorescence LOH 250350450550650750850 OH (b) Why must a molecule that fluoresces also have an absorption spectrum?
Please dont forget energy level diagrams
Using energy level diagrams explain why in fluorescence spectroscopy, the wavelength of the electromagnetic radiation emitted is longer than the wavelength absorbed.
Decide if the following statement Is true or false. Explain why. It is possible to have a standard deviation of 440, 000
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