Homework Answers
Reverse genetics is defined as what phenotype is responsible due to a particular genetic sequence. So if we are knocking a gene which is responsible for purring in cats by RNAi and then noticing the effect of such on purring ability then this is an example of reverse genetics.
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In order to investigate how cats are able to purr, you test a candidate gene by...
1. A scientist mutates a gene in yeast and then looks to see what effect the...
1. A scientist mutates a gene in yeast and then looks to see what effect the mutation has on the phenotype in yeast. This is an example of A. DNA fingerprinting. B. next-generation sequencing. C. RNAi. D. reverse genetics. E. positional cloning. 2. The increased levels of telomerase associated with many tumor cells likely promotes cancer by… A. Enhancing levels of DNA repair so that cells remain normal and have stable genomes and thus would be able to replicate their...
How can you prove that your chosen candidate is the CFTR gene? Select the three correct...
How
can you prove that your chosen candidate is the CFTR gene? Select
the three correct answers.
Problem 16.15 Mutations in the CFTR gene result in cystic fibrosis in humans, a condition in which abnormal secretions are present in the lungs, pancreas, and sweat glands. In the effort to positionally clone the CFTR gene, the gene was mapped to a region of 500 kb on chromosome 7 containing three candidate genes. Part A Using your knowledge of the disease symptoms,...
To investigate transformer (tra) gene expression during sex determination in Drosophila, you perform in situ hybridization...
To investigate transformer (tra) gene expression during sex determination in Drosophila, you perform in situ hybridization (FISH) and immunostaining. Fill in the table with the results you expect to observe following the example given in the first row. Experiment Female embryos Male embryos Immunostaining with an antibody to Sxl protein stain positively no staining FISH with a probe to exon 2 of tra FISH with a probe to exon 3 of tra Immunostaining with an antibody to Tra protein Match...
Help with genetics short answer.. a) how would you set up a test cross to determine...
Help with genetics short answer.. a) how would you set up a test cross to determine if a gene was Y or X linked? show with a punnett square to answer the question. How would the outcome be different if the gene was sex limited?
how would you test if the genes for body color (B dominant over b) and wing...
how
would you test if the genes for body color (B dominant over b) and
wing shape (V dominant over v) were linked (close to each other on
the same chromosome)?
In tortoiseshell cats, a gene on X chromosome determines fur color. Explain why female but not male cats have mosaic fur, containing patches of orange and black color.
Draw (or arrange) only the items you need from the Lab Materials Page (picture below) in...
Draw (or arrange) only the items you need from
the Lab Materials Page (picture below) in the
order you would use them to make AND screen a gene library.
You will ‘draw’ the steps that use the Lab Materials in the correct
order to create and screen a gene library which has the assigned
gene clone. You will need to add some connecting words and phrases
to make all the steps for making your library clear.
The genomic gene clone...
Please, can you give me you feedback on the following steps? In order to investigate how...
Please, can you give me you feedback on the following steps? In order to investigate how salt concentration will affect the specific heat capacity I elaborated the following experiment. I measured the amount of water and I record the initial temperature. I take an amount x of salt and blend it in the water. I heat the water for 2 minutes and then I measure the final temperature. I do it for the water with salt and without salt. Then I...
If you were able to collect a solid sample from test tube #7, and completely dry the crystals, how could you test your product for purity? The test tube has salicylic acid and methyl alcohol
If you were able to collect a solid sample from test tube #7, and completely dry the crystals, how could you test your product for purity? The test tube has salicylic acid and methyl alcohol
Gene: sax-3 Question: Test a different neuron to see how wide spread your function is: You...
Gene: sax-3 Question: Test a different neuron to see how wide spread your function is: You just found that your protein is involved in neural development in PHB neurons. To test if this is a wide-spread effect, you can label other neurons in the animal using a transgenic array, and introduce the transgenic array into your mutant. 1. Explain the approach and why it would be useful? 2. Show Genetic Crosses You would do in C. elegans to introduce the...
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
How
can you prove that your chosen candidate is the CFTR gene? Select
the three correct answers.
Problem 16.15 Mutations in the CFTR gene result in cystic fibrosis in humans, a condition in which abnormal secretions are present in the lungs, pancreas, and sweat glands. In the effort to positionally clone the CFTR gene, the gene was mapped to a region of 500 kb on chromosome 7 containing three candidate genes. Part A Using your knowledge of the disease symptoms,...
how
would you test if the genes for body color (B dominant over b) and
wing shape (V dominant over v) were linked (close to each other on
the same chromosome)?
In tortoiseshell cats, a gene on X chromosome determines fur color. Explain why female but not male cats have mosaic fur, containing patches of orange and black color.
Draw (or arrange) only the items you need from
the Lab Materials Page (picture below) in the
order you would use them to make AND screen a gene library.
You will ‘draw’ the steps that use the Lab Materials in the correct
order to create and screen a gene library which has the assigned
gene clone. You will need to add some connecting words and phrases
to make all the steps for making your library clear.
The genomic gene clone...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
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