Question

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You have determined a relationship between the growth (cell concentration numbers) of BL21 and optical density. You determined it to be 1 AU at 600 nm = 1 x 109 cells/ml. You wanted to use this relationship (calibration curve) for other cell concentrations (so you could use OD to determine cell concentrations), Which of the following cells could this relationship ( 1 AU at 600 nm = 1 x 109 cells/ml) be used for? O a. any E. coli strain b. any bacterial cell culture O c. any microbial strain Od. Candid albicans Oe. None of the above Of. Any of a - d

Answer 1

1. BL 21 is a strain of E. Coli. So it is applicable to E. Coli strains and may be applicable to bacilli of bacterial culture because E.Colis a bacilli.

Any microbial culture means may account for fungal cells which differs with cell concentration of bacteria. Candid albicans is a fungi.