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You have added a pure solution of formin to a solution of actin in ionic conditions...
An explanation would be appreciated. Thank you! Compared to the normal situation, in which actin monomers...
An explanation would be appreciated. Thank you!
Compared to the normal situation, in which actin monomers carry ATP, what do you predict would happen if actin monomers that bind a nonhydrolyzable form of ATP were incorporated into actin filaments? (a) Actin filaments would grow longer. (b) Actin filaments would grow shorter because depolymerization would be enhanced. (c) Actin filaments would grow shorter because new monomers could not be added to the filaments. (d) No change, as addition of monomers binding...
1. If you add short actin filaments marked by bound myosin heads (myosin-decorated filaments) to a...
1. If you add short actin filaments marked by bound myosin heads
(myosin-decorated filaments) to a solution with an excess of actin
monomers, wait for a few minutes, and then examine the filaments by
electron microscopy, you see the picture shown in Figure 16-5.
A. Which is the plus end of the myosin-decorated filaments and
which is the minus end? Which is the "barbed" end and which is the
"pointed" end? How can you tell?
B. If you diluted the...
Isolated bundles of actin filaments from the acrosomal processes of Limuluspolyphemus(horseshoe crab) sperm have readily distinguishable...
Isolated bundles of actin filaments from the acrosomal processes
of Limuluspolyphemus(horseshoe crab) sperm have readily
distinguishable plus ends (tapered) and minus ends
(blunt). Assembly at the ends of such bundles was used
to determine the mechanism of action of phalloidin, which has a
marked effect on actin assembly. When phalloidin is mixed with
actin in a molar ratio of at least 1:1, the growth rate increases
at both ends, as shown for minus ends in Fig.
16-11A. Because growth rate = kon[actin]...
Question 4 (1 point) You have discovered a new type of protein in bacteria that polymerizes...
Question 4 (1 point) You have discovered a new type of protein in bacteria that polymerizes into filaments similar to actin and microtubules. Through careful experiments you determine that the critical concentration of the minus end of the filament is 10 uM and the critical concentration of the plus end is 1 uM. If the soluble concentration of free unassembled protein is 5 uM what happens? O Polymerization at plus end and depolymerization at minus end? O Polymerization at plus...
Question 1 of 8 2.0 Points Which of the following statements about the cytoskeleton is false?...
Question 1 of 8 2.0 Points Which of the following statements about the cytoskeleton is false? A. The cytoskeleton is made up of three types of protein filaments. B. The cytoskeleton controls the location of organelles in eukaryotic cells. C. Covalent bonds between protein monomers hold together cytoskeletal filaments. D. The cytoskeleton of a cell can change in response to the environment. Reset Selection Question 2 of 8 2.0 Points Which of the following statements about the function of the...
2) You are studying actin polymerization in a solution. You perform an experiment where you add...
2) You are studying actin polymerization in a solution. You perform an experiment where you add free actin, and wait a sufficiently long time. You notice that at some concentration of added actin, increasing the concentration of actin beyond that point increases the average actin filament mass in grams/mL A) The point at which actin filament mass increases is the critical concentration B) dPol/dt=0, so free actin concentration is the same across the entire range of added concentrations. C) Above...
A.Microtubules can be assembled and disassembled quickly in response to the cellular changes is because: Choose...
A.Microtubules can be assembled and disassembled quickly in response to the cellular changes is because: Choose one answer Answers: 1. Cells synthesize tubulin proteins very fast. 2. Cells can turn other cytoskeletal filaments into microtubules 3. Microtubules have an instable plus end and microtubules are probably organized by weak non-covalent interactions 4. The motor proteins of microtubules can organize the microtubules in response to the cellular changes B.Kinesin is the motor protein that coorporates with microtubules. It has the following...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated...
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...
Hi, could you please help me by providing an accurate worked solution for the following problem. I have included the answers at the bottom of the page. I will leave you feedback that reflects the qual...
Hi,
could you please help me by providing an accurate worked
solution for the following problem. I have included the answers at
the bottom of the page. I will leave you feedback that reflects the
quality of your answer.
Thank you kindly
Consider a member wih a rectangular cross sechion subjected to a Campressive Icad p which is delivered tragh a rigid block on me upper end of me membe as illu stated belon Tne uper end of hhe member...
An explanation would be appreciated. Thank you!
Compared to the normal situation, in which actin monomers carry ATP, what do you predict would happen if actin monomers that bind a nonhydrolyzable form of ATP were incorporated into actin filaments? (a) Actin filaments would grow longer. (b) Actin filaments would grow shorter because depolymerization would be enhanced. (c) Actin filaments would grow shorter because new monomers could not be added to the filaments. (d) No change, as addition of monomers binding...
1. If you add short actin filaments marked by bound myosin heads
(myosin-decorated filaments) to a solution with an excess of actin
monomers, wait for a few minutes, and then examine the filaments by
electron microscopy, you see the picture shown in Figure 16-5.
A. Which is the plus end of the myosin-decorated filaments and
which is the minus end? Which is the "barbed" end and which is the
"pointed" end? How can you tell?
B. If you diluted the...
Isolated bundles of actin filaments from the acrosomal processes
of Limuluspolyphemus(horseshoe crab) sperm have readily
distinguishable plus ends (tapered) and minus ends
(blunt). Assembly at the ends of such bundles was used
to determine the mechanism of action of phalloidin, which has a
marked effect on actin assembly. When phalloidin is mixed with
actin in a molar ratio of at least 1:1, the growth rate increases
at both ends, as shown for minus ends in Fig.
16-11A. Because growth rate = kon[actin]...
Question 4 (1 point) You have discovered a new type of protein in bacteria that polymerizes into filaments similar to actin and microtubules. Through careful experiments you determine that the critical concentration of the minus end of the filament is 10 uM and the critical concentration of the plus end is 1 uM. If the soluble concentration of free unassembled protein is 5 uM what happens? O Polymerization at plus end and depolymerization at minus end? O Polymerization at plus...
Hi,
could you please help me by providing an accurate worked
solution for the following problem. I have included the answers at
the bottom of the page. I will leave you feedback that reflects the
quality of your answer.
Thank you kindly
Consider a member wih a rectangular cross sechion subjected to a Campressive Icad p which is delivered tragh a rigid block on me upper end of me membe as illu stated belon Tne uper end of hhe member...
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