Homework Answers
The answer for the above question is false because if you have a cup of water and you also have bacteria of a particular concentration , even if a cup of water is taken the concentration of the remaining water with bacteria remains the same that is the concentration of the bacteria remains the same that is 10^-4.
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D Question 9 2 pts You have 10-4 colonies/mL (concentration) of bacteria and you plate out...
You dilute a natural sample 10-4, plate 100 microliters, and observe 200 colonies on the plate....
You dilute a natural sample 10-4, plate 100 microliters, and observe 200 colonies on the plate. what was the initial concentration, in units of cells/ml?
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution...
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution of bacteria. How many CFU per ml of bacteria were in the uridiluted stock? (1 point)
You have a stock of an E. coli cell culture of an unknown concentration. You want...
You have a stock of an E. coli cell culture of an unknown concentration. You want to set up a serial dilution experiment so that you can count the viable colonies and determine the concentration of the stock in cells/ml. In tube A, you add 1 ml of the stock to 999 ml of media. In tube B, you add 1 ml of the material from tube A to 24 ml of media. In tube C, you add 50 microliters...
0.2 ml of a 10-9 dilution of a bacterial suspension plated on an agar plate gave...
0.2 ml of a 10-9 dilution of a bacterial suspension plated on an agar plate gave rise to 156 distinct colonies. What is the concentration (CFU/ml) of bacteria in the original, undiluted suspension?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete...
1. You have been given a tube that contains 2.4 x 109
bacteria/ml. Diagram a complete dilution scheme so that you will
have a plate with 240 colonies on it.
How would you change the scheme you have drawn above so that the
plate had 100 colonies on it?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete dilution scheme so that you will have a plate with 240 colonies on it. (3...
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24...
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8)...
You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24...
You spread 0.1 mL volume of a 10^(-5) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 223 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-7)...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
2 pts Question 8 In bacteria genes can be transcribed in operons. True False 2 pts...
2 pts Question 8 In bacteria genes can be transcribed in operons. True False 2 pts Question 9 In bacteria introns are present in most mRNA. True o False
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1....
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
4. You count 97 colonies on a plate inoculated with 2.5 ml of a 10* dilution of bacteria. How many CFU per ml of bacteria were in the uridiluted stock? (1 point)
1. You have been given a tube that contains 2.4 x 109
bacteria/ml. Diagram a complete dilution scheme so that you will
have a plate with 240 colonies on it.
How would you change the scheme you have drawn above so that the
plate had 100 colonies on it?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete dilution scheme so that you will have a plate with 240 colonies on it. (3...
2 pts Question 8 In bacteria genes can be transcribed in operons. True False 2 pts Question 9 In bacteria introns are present in most mRNA. True o False
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
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