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Thats the whole question provided. What other information do you need? 3. A human gene was...
3. A human gene was initially identified as having three exons and two introns. In order, the exons are 456, 224, and 524 bp, while the introns are 2.3 kb and 4.6 kb. a. Draw this gene showing the promoter, introns, exons, and the transcription start and stop sites. b. Surprisingly, it is found that this gene encodes not one but two mRNAs that have only 224 nucleotides in common. The original mRNA is 1204 nucleotides, while the new mRNA...
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
Question 2: a) You determine that xpd is expressed preferentially in muscle. Your colleague wants to know if any of the mutations affect the stability of the RNA transcript, thereby leading to higher turnover and lower overall mRNA levels. What techniques could you use to test her hypothesis? List the pros and cons of each method. (hint: try to think of at least three methods you could use) b) You find that your collegue is correct, and that the xpda...