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D 8.5-10.5. 10.5 -12.5. 12.5. If you were trying to separate histidine with values of I 80 (carboxyl group), 6.00 (R group 9.
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(10) PH expected to be basic 11.0 because in cation exchange chromatography positively charged proteins(cations) are attracted to the negatively charged solid media. (11)Ala-Met-Lys-Cys-Phe-Trp. because according to 2nd statement treatment with carboxypeptidase result in C-terminal lysine which is a basic compound.

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