Question

answer d and e

d) 2-0-methyl-purines and 2-fluoro-pyrimidines were used in the selection for the RNA aptamer drug Mucagen. Draw a 2-O-methyl-guanosine. Explain why this modification is important for stability of the drug. (6 points) e) Briefly describe how to use in vitro selection to make an RNA aptamer that binds tryptophan. (6 points)

Answer 1

Aptamers are single stranded DNA/RNA molecules that are capable of tight and specific binding with molecular or supramolecular targets.

→ STRUCTURE OF 2'-o-methylguanosine. Guanosine with the hydrogen on the hydroxyl group at position c-2 substituted with a methyl group. OD Macagen is a highly modified RNA aptamers. Aptamers are short sungle stranded RNA / DNA molecules that bind to the specific target. Inece aptamers are highly specific and are modified to prevent their degradation by nucleases - Currently, available modified aptososrs nucleotides are in -corporated into aptamers by engineered polymerases. These nucleotides are modified at 2' position by 20 methye, a'f , 24. NH₂ , which leads to the change in RNA structure flexibility. Note :- The structural stability of aptamers incorporated with rucently developed modified nucleotides semaine to be validated