Question

It was shown that 350 nucleotides in the 3'UTR of VG1 are required for its localization to the vegetal pole. Which of the following RNA fusions (see below) would you express in the oocyte to test whether these 350 nucleotides are sufficient for VG1 translocation to the vegetal pole? Justify your answer.

all the mRNAs have a 5'cap and a polyA tail

It was shown that 350 nucleotides in the 3'UTR of VG1 are required for its localization to the vegetal pole. Which of the following RNA fusions (see below) would you express in the oocyte to test whether these 350 nucleotides are sufficient for VG1 translocation to the vegetal pole? Justify your answer. all the mRNAs have a 5'cap and a polyA tail 5' U IT VGI ORE 350 nt3 VGI mRNA 5' - GD ORF GD mRNA A 5' TTTTTTTT VGI ORF VGI ORF ww 5' — VGI ORF 350 nt GD ORF 350 nt 3 D 5' — E5' TIM GD ORF GD ORF

Answer 1

I would suggest using the construct (C) for testing weather 350 nts in the 3' end is sufficient for the translocation to vegetal pole .It is the only construct that contains a 350 ntsequence that immediately follows the VG1 ORF. The consstruct A and B doesnt have a350 nt sequence following the ORF .The sequence D and E doesnt have the VG1 ORF in the construct,they have GD ORF in their construct.